Abstract
HUMAN pancreatic transplantation results, using the whole organ, have been disappointing and interest has been focused recently on the promising results obtained by transplanting isolated endocrine islets. Islet cell isotransplantation can restore the diabetic animal successfully to a state of normoglycaemia1,2. Unfortunately, islet allografts have been associated with only brief periods (4–8 d) of normoglycaemia, and attempts to prolong function with immunosuppressive therapy of the host have generally been unsuccessful3. The present results, however, suggest that the survival time of pancreatic islets, transplanted directly into the liver of a histoincompatible recipient, is prolonged by in vitro culture of the tissue before transplantation. Complete or partial control lasting up to more than 160d was achieved in 70% of recipients. Nevertheless, allogeneic cultured transplants are less effective in equivalent quantities than isogeneic grafts, in reversing an experimental diabetic state in rats.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ballinger, W. F. & Lacy, P. E. Surgery 72, 175–186 (1972).
Eloy, R. et al. Horm. metab. Res. 9, 40–46 (1977).
Finch, D. R. A. & Morris, P. J. Transplantation 22, 503–512 (1976).
Billingham, R. E. J. invest. Derm. 67, 149–159 (1976).
Lafferty, K. J., Colley, M. A., Woolnough, J. & Walker, K. Z. Science 188, 259–261 (1975).
Bach, F. H., Bach, M. L. & Sondel, P. M. Nature 259, 273–281 (1976).
Opelz, G. & Terasaki, P. I. Science 184, 464–466 (1974).
Stone, H. B., Owings, J. C. & Gey, G. O. Ann. Surg. 199, 613–626 (1934).
Gaillard, P. J. Ciba Fdn Symp. 100–106 (Churchill, London, 1964).
Ninnemann, J. L. & Good, R. A. Transplantation 18, 1–5 (1974).
Jacobs, B. B. Transplantation 18, 454–457 (1974).
Lafferty, K. J., Colley, M. A., Woolnough, J. & Warier, K. Z. Science 188, 259–261 (1975).
Boyles, R. R. & Seltzer, H. S. Diabetes (Abstr.) 24, 420 (1975).
Kedinger, M., Moody, A. J., Launay, J. F. & Haffen, K. Experientia 33, 972–974 (1977).
Eloy, R., Vuitton, D., Vaultier, J. P., Pousse, A. & Grenier, J. F. Cell. Immun. 21, 236–242 (1975).
Vuitton, D., Eloy, R., Coumaros, G. & Grenier, J. F. Cell. Immun. 28, 51–58 (1977).
Vuitton, D., Eloy, R., Clendinnen, G. & Grenier, J. F. Cell. Immun. (in the press).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
KEDINGER, M., HAFFEN, K., GRENIER, J. et al. In vitro culture reduces immunogenicity of pancreatic endocrine islets. Nature 270, 736–738 (1977). https://doi.org/10.1038/270736a0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/270736a0
- Springer Nature Limited