Skip to main content
SpringerLink
Log in

In vitro synthesis of infectious DNA of murine leukaemia virus

  • Article
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

DNA synthesised in vitro by purified virions of murine leukaemia virus is infectious. Neither RNA nor protein is required for infectivity. Transfection with reverse transcriptase product shows a single-hit dose response and results in the production of complete, infectious virus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Baltimore, D. Nature 226, 1209–1211 (1970).

    Article  ADS  CAS  Google Scholar 

  2. Temin, H. M. & Mizutani, S. Nature 226, 1211–1213 (1970).

    Article  ADS  CAS  Google Scholar 

  3. Boettiger, D. & Temin, H. M. Nature 228, 622–624 (1970).

    Article  ADS  CAS  Google Scholar 

  4. Balduzzi, P. & Morgan, H. R. J. Virol. 5, 470–477 (1970).

    Article  CAS  Google Scholar 

  5. Junghans, R. P., Duesberg, P. H. & Knight, C. A. Proc. natn. Acad. Sci. U.S.A. 72, 4895–4899 (1975).

    Article  ADS  CAS  Google Scholar 

  6. Rothenberg, E. & Baltimore, D. J. Virol. 21, 168–178 (1977).

    Article  CAS  Google Scholar 

  7. Hu, S., Davidson, N. & Verma, I. M. Cell 10, 469–477 (1977).

    Article  CAS  Google Scholar 

  8. Haseltine, W. A., Kleid, D. G., Panet, A., Rothenberg, E. & Baltimore, D. J. molec. Biol. 106, 109–131 (1976).

    Article  CAS  Google Scholar 

  9. Peters, G., Harada, F., Dahlberg, J. E., Panet, A., Haseltine, W. A. & Baltimore, D. J. Virol. 21, 1031–1041 (1977).

    Article  CAS  Google Scholar 

  10. Graham, F. L. & van der Eb, A. J. Virology 52, 456–467 (1973).

    Article  CAS  Google Scholar 

  11. Graham, F. L. & van der Eb, A. J. Virology 54, 536–539 (1973).

    Article  CAS  Google Scholar 

  12. Smotkin, D., Gianni, A. M., Rozenblatt, S. & Weinberg, R. A. Proc. natn. Acad. Sci. U.S.A. 72, 4910–4913 (1975).

    Article  ADS  CAS  Google Scholar 

  13. Smotkin, D., Yoshimura, F. K. & Weinberg, R. A. J. Virol. 20, 621–626 (1976).

    Article  CAS  Google Scholar 

  14. Rowe, W. P., Pugh, W. E. & Hartley, J. Virology 42, 1136–1139 (1970).

    Article  CAS  Google Scholar 

  15. Levy, J. A. Science 182, 1151–1153 (1973).

    Article  ADS  CAS  Google Scholar 

  16. Birnie, G. D. FEBS Lett. 26, 19–22 (1972).

    Article  Google Scholar 

  17. Manly, K. F., Smoler, D. F., Bromfeld, E. & Baltimore, D. J. Virol. 7, 106–111 (1971).

    Article  CAS  Google Scholar 

  18. Garapin, A.-C., Varmus, H. E., Faras, A. J., Levinson, W. E. & Bishop, J. M. Virology 52, 264–274 (1973).

    Article  CAS  Google Scholar 

  19. McDonell, M. W., Simon, M. N. & Studier, F. W. J. molec. Biol. 110, 119–146 (1977).

    Article  CAS  Google Scholar 

  20. Gianni, A. M. & Weinberg, R. A. Nature 255, 646–648 (1975).

    Article  ADS  CAS  Google Scholar 

  21. Fritsch, E. & Temin, H. M. J. Virol. 21, 119–130 (1977).

    Article  CAS  Google Scholar 

  22. Sharp, P. A., Sugden, B. & Sambrook, J. Biochemistry 12, 3055–3063 (1973).

    Article  CAS  Google Scholar 

  23. Laskey, R. A., Mills, A. D. & Knowland, J. S. Eur. J. Biochem. 56, 335–341 (1975).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biology and Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139

    Ellen Rothenberg, David Smotkin, David Baltimore & Robert A. Weinberg

Authors
  1. Ellen Rothenberg
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. David Smotkin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. David Baltimore
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Robert A. Weinberg
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rothenberg, E., Smotkin, D., Baltimore, D. et al. In vitro synthesis of infectious DNA of murine leukaemia virus. Nature 269, 122–126 (1977). https://doi.org/10.1038/269122a0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/269122a0

  • Springer Nature Limited

This article is cited by

Search

Navigation