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Synthetic lacoperator DNA is functional in vivo

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Abstract

Blunt end ligation with an EcoRI/ octanucleotide “linker” was used to construct a plasmid containing chemically synthesized lac operator DNA. This plasmid renders the host bacterium constitutive for β-galactosidase.

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Authors and Affiliations

  1. University of California, San Francisco, California, 94143

    Herbert L. Heyneker, John Shine, Howard M. Goodman & Herbert W. Boyer

  2. California Institute of Technology, Pasadena, California, 91106

    John Rosenberg & Richard E. Dickerson

  3. National Research Council of Canada, Ottawa, Ontario, K1A OR6, Canada

    Saran A. Narang

  4. City of Hope National Medical Center, Duarte, California, 91010

    Keiichi Itakura, Syr-yaung Lin & Arthur D. Riggs

Authors
  1. Herbert L. Heyneker
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  2. John Shine
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  3. Howard M. Goodman
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  4. Herbert W. Boyer
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  5. John Rosenberg
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  6. Richard E. Dickerson
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  7. Saran A. Narang
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  8. Keiichi Itakura
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  9. Syr-yaung Lin
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  10. Arthur D. Riggs
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Heyneker, H., Shine, J., Goodman, H. et al. Synthetic lacoperator DNA is functional in vivo. Nature 263, 748–752 (1976). https://doi.org/10.1038/263748a0

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  • DOI: https://doi.org/10.1038/263748a0

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