Skip to main content
SpringerLink
Log in

Preparation and characterisation of an antiserum to the mouse candidate for immunoglobulin D

  • Letter
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

ANALYSIS of mouse B-lymphocyte surface immunoglobulin (Ig) by isotopic labelling techniques reveals little, if any, IgA or IgG. Instead, the surface Ig consists of monomeric IgM and an Ig similar in physicochemical properties to human IgD1–3. In the absence of sequence comparison between human IgD and its assumed mouse counterpart, this assignment is tentative. With this proviso, however, and for the sake of clarity in presentation, we refer to this immunoglobulin as IgD. During development, IgM-bearing cells are the first to arise and the ratio of IgM–IgD determined with labelled adult lymphocytes is higher in the spleen than in lymph nodes or Peyer's patches1,4. These findings, however, give the total yield of the two Ig classes and not their distribution on individual lymphocytes. On the basis of the absence of immunoglobulins other than IgM or IgD on the surface of B lymphocytes, we indicated the presence of cells bearing IgM or IgD, or IgM and IgD in mouse spleen cell suspensions5,6. In these experiments, IgM was first capped with rhodamine-labelled anti-μ and subsequent ring staining with fluorescein-labelled anti-Fab in the presence of sodium azide was taken as evidence for the presence of IgD. We report here the preparation of an antibody specific for mouse IgD, and its use in the characterisation of mouse B-lymphocyte surface Ig.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Abney, E. R., and Parkhouse, R. M. E., Nature, 252, 600–602 (1974).

    Article  ADS  CAS  PubMed  Google Scholar 

  2. Melcher, U., et al., J. exp. Med., 140, 1427–1431 (1974).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Vitetta, E. S., et al., J. exp. Med., 141, 206–215 (1975).

    Article  CAS  PubMed  Google Scholar 

  4. Vitetta, E. S., McWilliams, M., Philips-Quagliata, J. M., Lamm, M. E., and Uhr, J. W., J. Immun., 115, 603–605 (1975).

    CAS  PubMed  Google Scholar 

  5. Parkhouse, R. M. E., Hunter, I. R., and Abney, E. R., Immunology (in the press).

  6. Parkhouse, R. M. E., and Abney, E. R., in Membrane Receptors of Lymphocytes (edit. by Seligmann, M. Preud'homme, J-L. and Kourilsky, F. M.), 51–56 (Masson, Paris, 1975).

    Google Scholar 

  7. Crumpton, M. J., and Snary, D., Contemp. Topics molec. Immun., 3, 27–56 (1974).

    Article  CAS  Google Scholar 

  8. Axen, R., Porath, J., and Ernback, S., Nature, 214, 1302–1304 (1967).

    Article  ADS  CAS  PubMed  Google Scholar 

  9. Parkhouse, R. M. E., and Askonas, B. A., Biochem. J., 115, 163–169 (1969).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Cebra, J. J., and Goldstein, G., J. Immun., 95, 230–245 (1965).

    CAS  PubMed  Google Scholar 

  11. Raff, M. C., Immunology, 19, 637–650 (1970).

    CAS  PubMed  PubMed Central  Google Scholar 

  12. Julius, M. H., Simpson, E., and Herzenberg, L. A., Eur. J. Immun., 3, 645–649 (1973).

    Article  CAS  Google Scholar 

  13. Knapp, W., Bolhuis, R. L. H., Radl, J., and Hijmans, W., J. Immun., 111, 1295–1298 (1973).

    CAS  PubMed  Google Scholar 

  14. Rowe, D. S., Hug, K., Forni, L., and Pernis, B., J. exp. Med., 138, 965–972 (1973).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Jones, P. P., Craig, S. W., Cebra, J. J., and Herzenberg, L. A., J. exp. Med., 140, 452–469 (1974).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Salsano, P., Froland, S. S., Natvig, J. B., and Michaelsen, T. E., Scand J. Immun., 3, 841–846 (1974).

    Article  CAS  Google Scholar 

  17. Williamson, A. R., Nature, 231, 359–362 (1971).

    Article  ADS  CAS  PubMed  Google Scholar 

  18. Marchalonis, J. J., Cone, R. E., and Santer, V., Biochem. J., 124, 921–927 (1971).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Hunter, W. M., and Greenwood, F. C., Nature, 194, 495–496 (1962).

    Article  ADS  CAS  PubMed  Google Scholar 

  20. Knopf, P. M., Parkhouse, R. M. E., and Lennox, E. S., Proc. natn. Acad. Sci. U.S.A., 58, 2288–2295 (1967).

    Article  ADS  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. National Institute for Medical Research, Mill Hill, London, NW7 1AA, UK

    ERIKA R. ABNEY, I. R. HUNTER & R. M. E. PARKHOUSE

Authors
  1. ERIKA R. ABNEY
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I. R. HUNTER
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. M. E. PARKHOUSE
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

ABNEY, E., HUNTER, I. & PARKHOUSE, R. Preparation and characterisation of an antiserum to the mouse candidate for immunoglobulin D. Nature 259, 404–406 (1976). https://doi.org/10.1038/259404a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/259404a0

  • Springer Nature Limited

This article is cited by

Search

Navigation