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Replication control in a composite plasmid constructed by in vitro linkage of two distinct replicons

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Abstract

Although it carries two competent replication systems, a composite plasmid formed in vitro by linkage of the complete ColE1 and pSC101 plasmid replicons at their unique EcoRI endonuclease cleavage sites normally uses only the replication origin and functions of the ColE1 component. Restriction of ColE1 replication functions by DNA polymerase I deprivation results, however, in exclusive use of the pSC101 replication origin. When using the ColE1 replication system the composite plasmid is nevertheless incompatible with both the parent replicons. This suggests that a trans-dominant gene product is involved in plasmid incompatibility and supports negative control rather than positive control models for regulation of the initiation of DNA replication.

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References

  1. Kornberg, A., DNA Synthesis, 176–296 (Freeman, San Francisco, 1974).

    Google Scholar 

  2. Messer, W., Dankwarth, L., Tippe-Schindler, R., Womack, J. E., and Zahn, G., ICN-UCLA Winter Conferences: DNA Synthesis and its Regulation (in the press, 1975).

    Google Scholar 

  3. Kriegstein, H. J., and Hogness, D. S., Proc. natn. Acad. Sci. U.S.A., 71, 135–139 (1974).

    Article  ADS  CAS  Google Scholar 

  4. Kasamatsu, H., and Vinograd, J., Nature new Biol., 241, 103–105 (1973).

    Article  CAS  Google Scholar 

  5. Rownd, R. H., Perlman, D., and Goto, N., Microbiology-1974 (edit. by Schles-singer, D.), 76–94 (American Society for Microbiology, 1974).

    Google Scholar 

  6. Crosa, J. H., Luttropp, L. K., Hefron, F., and Falkow, S., Molec. gen. Genet., 140, 39–50 (1975).

    Article  CAS  Google Scholar 

  7. Timmis, K., Cabello, F., and Cohen, S. N., Proc. natn. Acad. Sci. U.S.A., 71, 4556–4560 (1974).

    Article  ADS  CAS  Google Scholar 

  8. Kingsbury, D. T., and Helinski, D. R., Biochem. biophys. Res. Commun., 41, 1538–1544 (1970).

    Article  CAS  Google Scholar 

  9. Bazaral, M., and Helinski, D. R., Biochemistry, 7, 3513–3519 (1968).

    Article  CAS  Google Scholar 

  10. Cohen, S. N., and Chang, A. C. Y., Proc. natn. Acad. Sci. U.S.A., 70, 1293–1297 (1973).

    Article  ADS  CAS  Google Scholar 

  11. Lovett, M. A., Katz, L., and Helinski, D. R., Nature, 251, 337–340 (1974).

    Article  ADS  CAS  Google Scholar 

  12. Inselburg, J., Proc. natn. Acad. Sci. U.S.A., 71, 2256–2259 (1974).

    Article  ADS  CAS  Google Scholar 

  13. Tomizawa, J-I., Sakakibara, Y., and Kakefuda, T., Proc. natn. Acad. Sci. U.S.A., 71, 2260–2264 (1974).

    Article  ADS  CAS  Google Scholar 

  14. Gross, J., and Monk, M., Nature, 224, 1166–1168 (1969).

    Article  ADS  CAS  Google Scholar 

  15. Nordström, K., Ingram, L. C., and Lundbäck, A., J. Bact., 110, 562–569 (1972).

    PubMed  Google Scholar 

  16. Kool, A. J., and Nijkamp, H. J. J., J. Bact., 120, 569–578 (1974).

    CAS  PubMed  Google Scholar 

  17. Uhlin, B. E., and Nordström, K., J. Bact., 124, 641–649 (1975).

    CAS  PubMed  Google Scholar 

  18. Timmis, K., Cabello, F., and Cohen, S. N., Proc. natn. Acad. Sci. U.S.A., 72, 2242–2246 (1975).

    Article  ADS  CAS  Google Scholar 

  19. Franklin, T. J., and Godfrey, A., Biochem. J., 94, 54–60 (1965).

    Article  Google Scholar 

  20. Franklin, T. J., and Rownd, R. H., J. Bact., 115, 235–242 (1973).

    CAS  PubMed  Google Scholar 

  21. Timmis, K., and Winkler, U., Molec. gen. Genet., 124, 207–217 (1973).

    Article  CAS  Google Scholar 

  22. Hershfield, V., Boyer, H. W., Yanofsky, C., Lovett, M. A., and Helinski, D. R., Proc. natn. Acad. Sci. U.S.A., 71, 3455–3459 (1974).

    Article  ADS  CAS  Google Scholar 

  23. Clewell, D. B., Yagi, Y., and Bauer, B., Proc. natn. Acad. Sci. U.S.A., 72, 1720–1724 (1975).

    Article  ADS  CAS  Google Scholar 

  24. Echols, H., J. Bact., 85, 262–268 (1963).

    CAS  PubMed  Google Scholar 

  25. Cohen, S. N., Chang, A. C. Y., Boyer, H. W., and Helling, R. B., Proc. natn. Acad. Sci. U.S.A., 70, 3240–3244 (1973).

    Article  ADS  CAS  Google Scholar 

  26. Chang, A. C. Y., and Cohen, S. N., Proc. natn. Acad. Sci. U.S.A., 71, 1030–1034 (1974).

    Article  ADS  CAS  Google Scholar 

  27. Jacob, F., Brenner, S., and Cuzin, F., Cold Spring Harb. Symp. quant. Biol., 28, 329–343 (1969).

    Article  Google Scholar 

  28. Pritchard, R. H., Barth, P. T., and Collins, J., Symp. Soc. Gen. Microbiol., 19, 263–267 (1969).

    Google Scholar 

  29. Pritchard, R. H., Chandler, M. G., and Collins, J., Molec. gen. Genet., 138, 143–155 (1975).

    Article  CAS  Google Scholar 

  30. Starlinger, P., and Saedler, H., Biochimie, 54, 177–185 (1972).

    Article  CAS  Google Scholar 

  31. Hedges, R. W., and Jacob, A., Molec. gen. Genet., 132, 31–40 (1974).

    Article  CAS  Google Scholar 

  32. Kopecko, D. J., and Cohen, S. N., Proc. natn. Acad. Sci. U.S.A., 72, 1373–1377 (1975).

    Article  ADS  CAS  Google Scholar 

  33. Heffron, F., Sublett, R., Hedges, R. W., Jacob, A., and Falkow, S., J. Bact., 122, 250–256 (1975).

    CAS  PubMed  Google Scholar 

  34. Bachman, B. J., Bact. Rev., 36, 525–557 (1972).

    Google Scholar 

  35. Greene, P. J., Betlach, M. D., Goodman, H. M., and Boyer, H. W., Methods in Molecular Biology (edit. by Wickner, R. B.), 9, 87–103 (Marcel Dekker, New York, 1974).

    Google Scholar 

  36. Kleinschmidt, A. K., Methods in Enzymology, XII (edit. by Colowick, S., and Kaplan, N.), 361–377 (Academic, New York, 1968).

    Google Scholar 

  37. Davis, R. W., Simon, M., and Davidson, N., Methods in Enzymology, 21, (edit. by Grossman, L., and Moldave, K.), 413–428 (Academic, New York, 1971).

    Google Scholar 

  38. Delius, H., Howe, C., and Kozinski, A. W., Proc. natn. Acad. Sci. U.S.A., 68, 3049–3053 (1971).

    Article  ADS  CAS  Google Scholar 

  39. Cohen, S. N., Chang, A. C. Y., and Hsu, L., Proc. natn. Acad. Sci. U.S.A., 69, 2110–2114 (1972).

    Article  ADS  CAS  Google Scholar 

  40. Timmis, K., J. Bact., 109, 12–20 (1972).

    CAS  PubMed  Google Scholar 

  41. Timmis, K., and Hedges, A. J., Biochim. biophys. Acta, 262, 200–207 (1972).

    Article  CAS  Google Scholar 

  42. Cooper, P., Genet. Res. Camb., 17, 151–159 (1971).

    Article  CAS  Google Scholar 

  43. Datta, N., and Hedges, R. W., J. gen. Microbiol., 77, 11–17 (1973).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Medicine, Stanford University School of Medicine, Stanford, California, 94305

    Felipe Cabello, Kenneth Timmis & Stanley N. Cohen

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  1. Felipe Cabello
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  2. Kenneth Timmis
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  3. Stanley N. Cohen
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Cabello, F., Timmis, K. & Cohen, S. Replication control in a composite plasmid constructed by in vitro linkage of two distinct replicons. Nature 259, 285–290 (1976). https://doi.org/10.1038/259285a0

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  • DOI: https://doi.org/10.1038/259285a0

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