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Enrichment of fungal mutants by selective cell-wall lysis

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Abstract

SEVERAL procedures for enrichment of fungal mutants are known and have been reviewed1,2 but they have many limitations. Here we describe a new technique based on selective cell-wall lysis, which has wide applicability and is highly efficient for isolating mutants differing from the wild-type strain in growth requirements.

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References

  1. Hopwood, D. A., in Methods in Microbiology (edit. by Norris, J. R., and Ribbons, D. W.), 3 A, 363 (Academic Press, London and New York, 1970).

    Google Scholar 

  2. Fincham, J. R. S., and Day, P. R., Fungal Genetics (Blackwell Scientific Publications, Oxford and Edinburgh, 1971).

    Google Scholar 

  3. Eddy, A. A., and Williamson, D. H., Nature, 179, 1252 (1957).

    Article  ADS  Google Scholar 

  4. Holter, H., and Ottolenghi, P., C. r. Trav. Lab. Carlsberg, 31, 409 (1960).

    CAS  PubMed  Google Scholar 

  5. Garcia Mendoza, C., and Villanueva, J. R., Nature, 195, 1326 (1962).

    Article  ADS  CAS  Google Scholar 

  6. Gutz, H., Genetics, 69, 317 (1971).

    CAS  PubMed  PubMed Central  Google Scholar 

  7. Wickerham, L. J., Tech. Bull. No., 1029 (United States Department of Agriculture 1951).

  8. Johnson, B. F., J. Bact., 95, 1169 (1968).

    CAS  PubMed  Google Scholar 

  9. Svoboda, A., and Smith, D. G., Z. allg. Mikrobiol., 12, 685 (1972).

    Article  CAS  Google Scholar 

  10. Poole, R. K., and Lloyd, D., Archs Mikrobiol., 88, 257 (1973).

    Article  CAS  Google Scholar 

  11. Foury, F., and Goffeau, A., J. gen. Mikrobiol., 75, 227 (1973).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Microbiology, Attila József University, H-6701, Szeged, PO Box 428, Hungary

    L. FERENCZY, M. SIPICZKI & M. SZEGEDI

Authors
  1. L. FERENCZY
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  2. M. SIPICZKI
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  3. M. SZEGEDI
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FERENCZY, L., SIPICZKI, M. & SZEGEDI, M. Enrichment of fungal mutants by selective cell-wall lysis. Nature 253, 46–47 (1975). https://doi.org/10.1038/253046a0

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  • DOI: https://doi.org/10.1038/253046a0

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