Summary
The effect of 2-deoxy-d-glucose on growth of Schizosaccharomyces pombe 972h−, and on its cell wall composition, structure and susceptibility to enzymatic degradation has been investigated. The growth rate was not markedly affected at below 80 μg/ml of the inhibitor, but the increased frequency of appearance of aberrant forms and the reduction in the final population attained varied directly with increased inhibitor concentration. These abnormal cells show localized lesions and, at these points, extrusion of cell contents still enclosed by an inner cell wall layer which is not sensitive to osmotic shock. Cells grown normally yield “prosphaeroplasts” on treatment with snail digestive enzymes; thiol pretreatment was not necessary but degradation proceeded more rapidly when MgSO4 was used in place of sorbitol as osmotic stabilizer. Deoxyglucose-grown organisms rapidly yield true osmotically-sensitive sphaeroplasts on similar treatment.
Analysis of isolated walls from mechanically disrupted cells showed marked changes in composition; an increase in the total lipid was accompanied by decreased content of both glucose and glucosamine.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bacon, J. S. D., Jones, D., Farmer, V. C., Webley, D. M.: The occurrence of α(1→3) glucan in Cryptococcus, Schizosaccharomyces and Polyporus species, and its hydrolysis by a Streptomyces culture filtrate lysing cell walls of Cryptococcus. Biochim. biophys. Acta (Amst.) 158, 313–315 (1968).
Bartnicki-Garcia, S., Nickerson, W. J.: Isolation, composition and structure of cell walls of filamentous and yeast-like forms of Mucor rouxii. Biochim. biophys. Acta (Amst.) 58, 102–119 (1962).
Biely, P., Krátký, Z., Bauer, S.: Incorporation of 2-deoxy-d-glucose into mannan of Saccharomyces cerevisiae. Abstract communicated at the 7th Meeting of the European Biochemical Societies, Verna, Abstract 585 (1971).
Biely, P., Krátký, Z., Kovařík, J., Bauer, S.: Effect of 2-deoxyglucose on cell wall formation in Saccharomyces cerevisiae and its relation to cell growth inhibition. J. Bact. 107, 121–129 (1971).
Birnboim, H. C.: New method for the extraction of ribonucleic acid and polyribosomes from Schizosaccharomyces pombe. J. Bact. 107, 659–663 (1971).
Clamp, J. R., Bhatti, T., Chambers, R. E.: The determination of carbohydrate in biological materials by gas-liquid chromatography. In: Methods of Biochemical Analysis, vol. 19, p. 229. Ed. by D. Glick. New York: Interscience Publishers 1971.
Duell, E. A., Inoue, S., Utter, M. R.: Isolation and properties of intact mitochondria from sphaeroplasts of yeast. J. Bact. 88, 1762–1773 (1964).
Gerhardt, P., Judge, J. A.: Porosity of isolated cell walls of Saccharomyces cerevisiae and Bacillus megaterium. J. Bact. 87, 945–951 (1964).
Havelková, M.: A comparative study of sub-microscopic structures of protoplasts of various yeast species. Folia microbiol. (Praha) 11, 453–458 (1966).
Holligan, P. M.: Routine analysis by gas-liquid chromatography of soluble carbohydrates in extracts of plant tissues. I. A review of the techniques used for the separation, identification and estimation of carbohydrates by gas-liquid chromatography. New Phytol. 70, 239–269 (1971).
Hughes, D. E.: A press for disrupting bacteria and other microorganisms. Brit. J. exp. Path. 32, 97–109 (1951).
Johnson, B. F.: Growth of the fission yeast, Schizosaccharomyces pombe with late, eccentric, lytic fission in an unbalanced medium. J. Bact. 94, 192–195 (1967).
Johnson, B. F.: Dissolution of yeast glucan induced by 2-deoxyglucose. Exp. Cell Res. 50, 692–694 (1968a).
Johnson, B. F.: Lysis of yeast cell walls induced by 2-deoxyglucose at their sites of glucan synthesis. J. Bact. 95, 1169–1172 (1968b).
Kogané, F., Yanagita, T.: Qualitative and quantitative studies on the cell wall carbohydrate in the yeast Schizosaccharomyces pombe. Chem. pharm. Bull. 10, 61–66 (1962).
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951).
Megnet, R.: Effect of 2-deoxyglucose on Schizosaccharomyces pombe. J. Bact. 90, 1032–1035 (1965).
Mitchison, J. M.: Physiological and cytological methods for Schizosaccharomyces pombe. In: Methods in Cell Physiology, vol. 4, pp. 131–165. Ed. by D. M. Prescott. New York: Academic Press Inc. 1970.
Nurminen, T., Oura, E., Suomalainen, H.: The enzymic composition of the isolated cell wall and plasma membrane of baker's yeast. Biochem. J. 116, 61–69 (1970).
Streiblová, E.: Surface structure of yeast protoplasts. J. Bact. 95, 700–707 (1968).
Tkacz, J. S., Cybulska, E. B., Lampen, J. O.: Specific staining of wall mannan in yeast cells with fluorescein-conjugated Concanavalin. J. Bact. 105 1–5 (1971).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Poole, R.K., Lloyd, D. Effect of 2-deoxy-d-glucose on growth and cell walls of Schizosaccharomyces pombe 972h− . Archiv. Mikrobiol. 88, 257–272 (1973). https://doi.org/10.1007/BF00409939
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00409939