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Very long stretches of free DNA in chromatin

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Abstract

THE arrangement of histones along the DNA in chromosomes has been studied using digestion of the chromatin with DNase1–4 and titration of the chromatin with polylysine2,5 or certain dyes3,6 as probes. The precision of these probes is limited by the extent to which DNA partially covered with histones will react with the enzymes or titration agents used7,8. Furthermore, it has been noted that histones are redistributed along or between DNA fibres under conditions arising during the normal course of isolation and handling of the chromatin9–12. This phenomenon may have strongly influenced the results obtained previously.

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Authors and Affiliations

  1. Institute of Molecular Biology, Academy of Sciences of the USSR, Moscow, USSR

    A. J. VARSHAVSKY, Yu. V. ILYIN & G. P. GEORGIEV

Authors
  1. A. J. VARSHAVSKY
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  2. Yu. V. ILYIN
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  3. G. P. GEORGIEV
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VARSHAVSKY, A., ILYIN, Y. & GEORGIEV, G. Very long stretches of free DNA in chromatin. Nature 250, 602–606 (1974). https://doi.org/10.1038/250602a0

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