Abstract
THE in situ DNA-RNA hybridisation techniques to locate a certain genome fraction with unique nucleotide sequences1–6 have assigned ribosomal cistrons to the satellites of human D and G group chromosomes4,5. This is of particular interest because the satellites organise nucleoli in somatic7 and meiotic cells8–10. During the course of study on the Giemsa banding mechanism (unpublished) we found that the satellite bodies of human acrocentrics can be differentially stained with Giemsa after simple procedures including extraction of both nucleic acids and histones. The staining profile, herein referred to as ‘N band’, differed clearly from the Quinacrine, Giemsa, Reversed-Giemsa, Centromeric, or Giemsa 11 banding patterns11,12. In rat kangaroo chromosomes, the N bands appeared exclusively in the nucleolus organisers13,14, that is, in the secondary constriction of the X chromosome. Further application of this technique to other mammalian species strongly indicated that the N-band-positive sites coincide with nucleolus organisers.
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References
Pardue, M. L., and Gall, J. G., Proc. natn. Acad. Sci. U.S.A., 64, 600 (1969).
John, H. A., Birnstiel, M. L., and Jones, K. W., Nature, 223, 582 (1969).
Pardue, M. L., Gerbi, S. A., Eckhardt, R. A., and Gall, J. G., Chromosoma, 29, 268 (1970).
Henderson, A. S., Warburton, D., and Atwood, K. C., Proc. natn. Acad. Sci. U.S.A., 69, 3394 (1972).
Bross, K., and Krone, W., Humangenetik, 14, 137 (1972).
Price, P. M., Conover, J. H., and Hirschhorn, K., Nature, 237, 340 (1972).
Ohno, S., Trujillo, J. M., Kaplan, W. D., and Kinoshita, R., Lancet, ii, 123 (1961).
Ferguson-Smith, M. A., Cytogenetics, 3, 124 (1964).
Schultz, J., and St. Lawrence, P., J. Hered., 40, 30 (1949).
Hungerford, D. A., Cytogenetics, 10, 23 (1971).
Paris Conference (1971). Standardization in Human Cytogenetics. Birth Defects: Original Article Series, VIII, 7 (The National Foundation, New York, 1972).
Bobrow, M., Madan, K., and Pearson, P. L., Nature new Biol., 238, 122 (1972).
Hsu, T. C., Brinkley, B. R., and Arrighi, F. E., Chromosoma, 23, 137 (1967).
Ohnuki, Y., Olson, R. S., and Rounds, D. E., Jap. J. Genet., 47, 133 (1972).
Matsui, S., Yoshida, H., Weinfeld, H., and Sandberg, A. A., J. Cell Biol., 54, 120 (1972).
Takagi, N., and Oshimura, M., Expl Cell Res., 78, 127 (1973).
Caspersson, T., Zech, L., Johansson, C., and Modest, E. J., Chromosoma, 30, 215 (1970).
Wurster, D. H., and Benrischke, K., Science, N.Y., 168, 1364 (1970).
Sasaki, M., and Makino, S., Proc. Japan Acad., 47, 676 (1971).
Comings, D. E., Expl Cell Res., 67, 441 (1971).
Heneen, W. K., and Nichols, W. W., J. Cell Biol., 31, 543 (1966).
Hsu, T. C., Cooper, J. E., Mace, jun., M. L., and Brinkley, B. R., Chromosoma, 34, 73 (1971).
Smetana, K., and Busch, H., Cancer Res., 26, 331 (1966).
Alfert, M., and Geschwind, I. I., Proc. natn. Acad. Sci. U.S.A., 39, 992 (1953).
Black, M., and Ansley, H. R., J. Histochem. Cytochem., 14, 177 (1966).
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MATSUI, SI., SASAKI, M. Differential Staining of Nucleolus Organisers in Mammalian Chromosomes. Nature 246, 148–150 (1973). https://doi.org/10.1038/246148a0
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DOI: https://doi.org/10.1038/246148a0
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