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The Intercistronic Divide: Translation of an Intercistronic Region in the Histidine Operon of Salmonella typhimurium

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Abstract

A frameshift mutation near the end of the hisD gene of the histidine operon results in the production of an active hisD gene product. Analysis of the C-terminal sequence of the mutant protein establishes that the normal in vivo hisD chain termination codon can be UAA or UGA but not UAG, and demonstrates the existence of an Intercistronic region between two genes of the operon.

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References

  1. Steitz, J. A., Nature, 224, 957 (1969).

    Article  ADS  CAS  PubMed  Google Scholar 

  2. Martin, R. G., Ann. Rev. Genet., 3, 181 (1969).

    Article  CAS  Google Scholar 

  3. Ames, B. N., Goldberger, R. F., Hartman, P. E., Martin, R. G., and Roth, J. R., in Regulation of Nucleic Acid and Protein Biosynthesis (edit. by Koningsberger, V. V., and Bosch, L.), 272 (Elsevier, Amsterdam, 1967).

    Google Scholar 

  4. Martin, R. G., Cold Spring Harbor Symp. Quant. Biol., 28, 357 (1963).

    Article  CAS  Google Scholar 

  5. Whitfield, jun., H. J., Gutnick, D. L., Margolies, M. N., Martin, R. G., Rechler, M. M., and Voll, M. J., J. Mol. Biol., 49, 245 (1970).

    Article  CAS  PubMed  Google Scholar 

  6. Crick, F. H. C., Barnett, L., Brenner, S., and Watts-Tobin, R. J., Nature, 192, 1227 (1961).

    Article  ADS  CAS  PubMed  Google Scholar 

  7. Voll, M. J., J. Mol. Biol., 30, 109 (1967).

    Article  CAS  PubMed  Google Scholar 

  8. Fink, G. R., Klopotowski, T., and Ames, B. N., J. Mol. Biol., 30, 81 (1967).

    Article  CAS  PubMed  Google Scholar 

  9. Vogel, H. J., and Bonner, D. M., J. Biol. Chem., 218, 97 (1956).

    CAS  PubMed  Google Scholar 

  10. Loper, J. C., and Adams, E., J. Biol. Chem., 240, 788 (1965).

    CAS  PubMed  Google Scholar 

  11. Davis, B. J., Ann. NY Acad. Sci., 121, 404 (1964).

    Article  ADS  CAS  PubMed  Google Scholar 

  12. Ames, B. N., Hartman, P. E., and Jacob, F., J. Mol. Biol., 7, 23 (1963).

    Article  CAS  PubMed  Google Scholar 

  13. Martin, R. G., Silbert, D. F., Smith, D. W. E., and Whitfield, jun., H. J., J. Mol. Biol., 21, 357 (1966).

    Article  CAS  PubMed  Google Scholar 

  14. Martin, R. G., and Talal, N., J. Mol. Biol., 36, 219 (1968).

    Article  CAS  PubMed  Google Scholar 

  15. Atkins, J. F., and Loper, J. C., Proc. US Nat. Acad. Sci., 65, 925 (1970).

    Article  ADS  CAS  Google Scholar 

  16. Report of the Commission on Enzymes of the International Union of Biochemistry, 20, 10 (1961).

  17. Hilton, J. L., Kearney, P. C., and Ames, B. N., Arch. Biochem. Biophys., 112, 544 (1965).

    Article  CAS  PubMed  Google Scholar 

  18. Ames, B. N., and Whitfield, jun., H. J., Cold Spring Harbor Symp. Quant. Biol., 31, 221 (1966).

    Article  CAS  PubMed  Google Scholar 

  19. Voll, M. J., Appella, E., and Martin, R. G., J. Biol. Chem., 242, 1760 (1967).

    CAS  PubMed  Google Scholar 

  20. Martin, R. G., and Goldberger, R. F., J. Biol. Chem., 242, 1168 (1967).

    CAS  PubMed  Google Scholar 

  21. Ames, B. N., Garry, B., and Herzenberg, L. A., J. Gen. Microbiol., 22, 369 (1960).

    Article  CAS  PubMed  Google Scholar 

  22. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., J. Biol. Chem., 193, 265 (1961).

    Google Scholar 

  23. Yourno, J., and Ino, I., J. Biol. Chem., 243, 3273 (1968).

    CAS  PubMed  Google Scholar 

  24. Yourno, J., J. Biol. Chem., 243, 3277 (1968).

    CAS  PubMed  Google Scholar 

  25. Katz, A. M., Dreyer, W. J., and Anfinsen, C. B., J. Biol. Chem, 234, 2897 (1959).

    CAS  PubMed  Google Scholar 

  26. Ambler, R. P., in Methods in Enzymology: Enzyme Structure (edit. by Hirs, C. H. W.), 11, 155 (1967).

    Book  Google Scholar 

  27. Fraenkel-Conrat, H., and Tsung, C. M., Methods in Enzymology: Enzyme Structure (edit. by Hirs, C. H. W.), 11, 151 (1967).

    Book  Google Scholar 

  28. Nichols, J. L., Nature, 225, 147 (1970).

    Article  ADS  CAS  PubMed  Google Scholar 

  29. Lengyel, P., and Söll, D., Bact. Rev., 33, 264 (1969).

    CAS  PubMed  PubMed Central  Google Scholar 

  30. Martin, R. G., Voll, M. J., and Apella, E., J. Biol. Chem., 242, 1175 (1967).

    CAS  PubMed  Google Scholar 

  31. Gray, W. R., and Hartley, B. S., Biochem. J., 89, 59P (1963).

    Article  Google Scholar 

  32. Riordan, J. F., and Vallee, B. L., in Methods in Enzymology: Enzyme Structure (edit. by Hirs, C. H. W.), 11, 565 (1967).

    Book  Google Scholar 

  33. Stark, G. R., and Smyth, D. G., J. Biol. Chem., 238, 214 (1963).

    CAS  PubMed  Google Scholar 

  34. Sarabhai, A., and Brenner, S., J. Mol. Biol., 27, 145 (1967).

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Laboratory of Molecular Biology, National Institute of Arthritis and Metabolic Diseases, National Institutes of Health, Bethesda, Maryland, 20014

    MATTHEW M. RECHLER & ROBERT G. MARTIN

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  1. MATTHEW M. RECHLER
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  2. ROBERT G. MARTIN
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RECHLER, M., MARTIN, R. The Intercistronic Divide: Translation of an Intercistronic Region in the Histidine Operon of Salmonella typhimurium. Nature 226, 908–911 (1970). https://doi.org/10.1038/226908a0

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  • DOI: https://doi.org/10.1038/226908a0

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