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Generation of Random luxCDABE Transcriptional Fusions in the Genome of Salmonella enterica

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Salmonella

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2182))

Abstract

The luxCDABE operon of Photorhabdus luminescens can be used as a bioluminescent reporter to measure gene transcription nondestructively. Here we describe protocols to (1) generate random transcriptional fusions of the lux operon to genes of the Salmonella genome, (2) screen for specific fusions with constitutive expression, Salmonella pathogenicity island 1-related expression, or Salmonella pathogenicity island 2-related expression, and (3) determine the site of luxCDABE integration.

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Acknowledgments

The work in the laboratory is supported by grants SAF2016-75365-R from the Spanish Ministry of Science and Innovation and the European Regional Development Fund, and PID2019-106132RB-I00 from the Spanish Ministry of Science and Innovation. J.B.-B. was supported by the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 842629. We are grateful to Anja Wiechmann and Paul Williams (University of Nottingham) for the gift of plasmid pUTmini-Tn5luxCDABE.

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Authors and Affiliations

  1. Facultad de Biología, Departamento de Genética, Universidad de Sevilla, Sevilla, Spain

    Juan J. de la Rosa-Altura, Barbora Benesova, Concepción Panadero-Medianero, Aida Amador-Álvarez, Julia Aguilera-Herce, Juan Luis Araujo-Garrido, Joaquín Bernal-Bayard & Francisco Ramos-Morales

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  1. Juan J. de la Rosa-Altura
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  2. Barbora Benesova
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  3. Concepción Panadero-Medianero
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  4. Aida Amador-Álvarez
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  5. Julia Aguilera-Herce
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  6. Juan Luis Araujo-Garrido
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  7. Joaquín Bernal-Bayard
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  8. Francisco Ramos-Morales
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Corresponding author

Correspondence to Francisco Ramos-Morales .

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Editors and Affiliations

  1. Department of Veterinary Pathobiology, University of Missouri-Columbia, Columbia, MO, USA

    Heide Schatten

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de la Rosa-Altura, J.J. et al. (2021). Generation of Random luxCDABE Transcriptional Fusions in the Genome of Salmonella enterica. In: Schatten, H. (eds) Salmonella. Methods in Molecular Biology, vol 2182. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0791-6_13

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  • DOI: https://doi.org/10.1007/978-1-0716-0791-6_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0790-9

  • Online ISBN: 978-1-0716-0791-6

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