Abstract
DIMETHYLAMINONAPHTHYL sulphonyl chloride (dansyl chloride) has been used in recent years as an end group analytical reagent in the study of protein structure1 and in the determination of amino-acids2–4. Dansyl chloride reacts with primary and secondary amines and with phenolic hydroxyl groups4. Chlorpromazine, after administration to patients, yields over twenty known metabolites5. At least ten of these metabolites possess either an amino or a phenolic hydroxyl group, or both, as potential reactive sites for dansyl chloride coupling. We have studied the reactivity of dansyl chloride with the chlorpromazine metabolites listed in Table 1.
Similar content being viewed by others
References
Weber, G., Biochem. J., 51, 155 (1952).
Chen, R. F., Arch. Biochem. Biophys., 120, 609 (1967).
Pataki, G., and Wang, K. T., J. Chromatog., 37, 499 (1968).
Gray, W. R., in Methods in Enzymology, 11, 139 (Academic Press, New York, 1967).
Goldenberg, H., and Fishman, V., Proc. Soc. Exp. Biol. Med., 109, 178 (1961).
Forrest, F. M., and Forrest, I. S., Amer. J. Psychiat., 113, 931 (1957).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
KAUL, P., CONWAY, M. & CLARK, M. Sensitive Quantitative Determination of Chlorpromazine Metabolites. Nature 226, 372–373 (1970). https://doi.org/10.1038/226372a0
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1038/226372a0
- Springer Nature Limited
This article is cited by
-
Chlorpromazine metabolism in humans. Part I
Mikrochimica Acta (1973)