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Isolation of Chromatin-free RNA Polymerase from Mammalian Cell Nuclei

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Abstract

MAMMALIAN RNA polymerase1 has been obtained in a soluble form from tumours2,3, chick embryo4, rat testis5 and rat liver nuclei6,7. The extraction procedures used involved the disruption of cell nuclei by sonication or exposure either to a hypotonic solution or alkaline pH. As a consequence, extracts were grossly contaminated with nuclear DNA and other cellular components and the yields of the enzyme were usually very low. We describe here a procedure for the rapid extraction from liver nuclei of fairly large yields of soluble RNA polymerase with fairly high specific activity.

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Authors and Affiliations

  1. Ben May Laboratory for Cancer Research and Department of Biochemistry, University of Chicago, Chicago, Illinois

    SHUTSUNG LIAO, DAPHNA SAGHER & SEN-MAW FANG

Authors
  1. SHUTSUNG LIAO
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  2. DAPHNA SAGHER
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  3. SEN-MAW FANG
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LIAO, S., SAGHER, D. & FANG, SM. Isolation of Chromatin-free RNA Polymerase from Mammalian Cell Nuclei. Nature 220, 1336–1337 (1968). https://doi.org/10.1038/2201336a0

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  • DOI: https://doi.org/10.1038/2201336a0

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