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Protein Synthesis in Megaloblastic Erythropoiesis caused by Vitamin B12 Deficiency

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Abstract

LITTLE information is available about protein synthesis in the megaloblasts of pernicious anaemia. In experimental animals deprived of vitamin B12, however, depressed protein synthesis has been demonstrated both in vivo1 and in cell cultures and cell free systems derived from deficient animals1–3. The addition of cobamide co-enzymes to such in vitro systems enhances the incorporation of labelled amino-acids into proteins2,3, while the addition of vitamin B12 alone has little or no effect3,4. Following the recent demonstration of a pile-up of megaloblasts in the G2 stage of interphase in pernicious anaemia5, we decided to investigate the protein synthetic activity of these abnormal cells by autoradiography.

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Authors and Affiliations

  1. Department of Pathology, University of Cambridge,

    S. N. WICKRAMASINGHE & D. G. CHALMERS

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  1. S. N. WICKRAMASINGHE
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  2. D. G. CHALMERS
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WICKRAMASINGHE, S., CHALMERS, D. Protein Synthesis in Megaloblastic Erythropoiesis caused by Vitamin B12 Deficiency. Nature 218, 463–464 (1968). https://doi.org/10.1038/218463a0

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