Skip to main content
SpringerLink
Log in

Dissociation and Inhibition of the Stimulatory Effect of Phytohaemagglutinin on Protein and DNA Synthesis in Human Lymphocyte Cultures

  • Letter
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

PHYTOHAEMAGGLUTININ (PHA) stimulates both DNA1 and protein synthesis2 by human peripheral white blood cells in tissue culture. Evidence to be presented indicates that this phenomenon is partly dependent on serum factors. The maximum synthesis of these two macromolecules in stimulated cultures occurs at different concentrations of serum in the tissue culture medium. In addition, increases in the concentration of serum beyond that which provided for maximal synthesis resulted in inhibition of the PHA stimulation on both DNA and protein synthesis. Variation in the serum content of unstimulated cultures had no significant effect on the synthesis of these substances. These results were independent of the source of serum, that is, whether autologous, homologoiis or heterologous.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. McIntyre, O. R., and Ebaugh, jun., F. G., Blood, 19, 443 (1962).

    CAS  Google Scholar 

  2. Hirschhorn, K., Bach, F., Koloday, R. L., Firschein, I. L., and Hashem, N., Science, 142, 1185 (1963).

    Article  ADS  CAS  Google Scholar 

  3. Cooperband, S. R., Nahmias, A. J., and Kibrick, S. (in the press).

  4. Kahan, F. M., Anal. Biochem., 2, 107 (1960).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. V and VI Medical Service, Boston City Hospital,

    S. R. COOPERBAND, J. A. GREEN, M. A. KENNEDY & M. M. GRANT

  2. Departments of Medicine and Microbiology, Boston University Medical School, Boston, Massachusetts

    S. R. COOPERBAND, J. A. GREEN, M. A. KENNEDY & M. M. GRANT

Authors
  1. S. R. COOPERBAND
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. A. GREEN
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. A. KENNEDY
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. M. GRANT
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

COOPERBAND, S., GREEN, J., KENNEDY, M. et al. Dissociation and Inhibition of the Stimulatory Effect of Phytohaemagglutinin on Protein and DNA Synthesis in Human Lymphocyte Cultures. Nature 214, 1240–1241 (1967). https://doi.org/10.1038/2141240a0

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1038/2141240a0

  • Springer Nature Limited

This article is cited by

Search

Navigation