Abstract
WE reported earlier1 that the mature anthers of Datura produce numerous embryo-like structures when cultured on Nitsch's medium containing 15 per cent coconut milk. We suggested two possibilities for their origin: (1) from the connective tissue; (2) from the pollen grains. The precise situation, however, could not be determined at that time. We now report evidence which shows that these embryoids originate from the pollen grains.
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References
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GUHA, S., MAHESHWARI, S. Cell Division and Differentiation of Embryos in the Pollen Grains of Datura in vitro. Nature 212, 97–98 (1966). https://doi.org/10.1038/212097a0
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DOI: https://doi.org/10.1038/212097a0
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