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Isolation of Δ7-Cholesten-3-one from Butterfat

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Abstract

THE direct reaction of butterfat with 2,4-dinitrophenylhydrazine has resulted in the identification of a number of previously undescribed carbonyl compounds1,2 from dairy products. During the course of those investigations a 2,4-dinitrophenylhydrazone (hereafter abbreviated to DNPhydrazone) was observed with chromatographic properties similar to the aldehyde DNPhydrazones but differing in light absorption properties. Initial studies revealed that the unknown DNPhydrazone gave a positive response to the Liebermann-Burchard reaction. This communication presents some investigations in which the compound has been identified as the DNPhydrazone of Δ7-cholesten-3-one.

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References

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Authors and Affiliations

  1. Dairy Products Laboratory Agricultural Research Service, U.S. Department of Agriculture, Washington, D.C.

    OWEN W. PARKS & DANIEL P. SCHWARTZ

  2. Department of Dairy Science, University of Maryland, College Park, Maryland

    MARK KEENEY

  3. Division of Food Chemistry, Food and Drug Administration, Washington, D.C.

    JOSEPH N. DAMICO

Authors
  1. OWEN W. PARKS
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  2. DANIEL P. SCHWARTZ
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  3. MARK KEENEY
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  4. JOSEPH N. DAMICO
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PARKS, O., SCHWARTZ, D., KEENEY, M. et al. Isolation of Δ7-Cholesten-3-one from Butterfat. Nature 210, 416–417 (1966). https://doi.org/10.1038/210416a0

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