Abstract
A PREVIOUS investigation has shown that the isolated bladder of the toad, B. marinus, releases an inhibitor of oxytocin into the bathing Ringer's solution1. More recently a similar inhibitor has been invoked to explain the inhibition of the effects of vasopressin and of adenosine-3′, 5′-monophosphate on the toad bladder2. In the former investigation it was shown that inhibitor could be obtained by gently stirring isolated toad bladders in a modified Ringer's solution, and that the inhibitor so obtained was not readily dialysable. In the investigation reported here the previous results were confirmed; however, it was found that, following heat-treatment of the inhibitor solution, the inhibitor became dialysable. We report a modified preparation of the inhibitor based on this finding.
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References
Karlin, A., Biochem. Biophys. Res. Comm., 11, 44 (1963).
Edelman, I. S., Petersen, M. J., and Gulyassy, P. F., J. Clin. Invest., 43, 2185 (1964).
Bentley, P. J., J. Endocrinol., 17, 201 (1958).
Craig, L. C., Science, 144, 1093 (1964).
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KARLIN, A., OVERWEG, N. An Inhibitor of Oxytocin from the Urinary Bladder of the Toad, Bufo marinus. Nature 207, 1401–1402 (1965). https://doi.org/10.1038/2071401b0
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DOI: https://doi.org/10.1038/2071401b0
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