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Effects of 4-Benzyloxy-3,5-diiodo-benzoic Acid on the Oxidative Phosphorylation of Isolated Mitochondria

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Abstract

WE have investigated the influence of 4-benzyloxy-3,5-diiodo-benzoic acid (BIBA) on the oxidative phosphoryl-ation of isolated rat liver mitochondria. The work continues previous investigations from our laboratory regarding the effects of chlorpromazine1, librium2, L-3,5,3′-triiodo-thyronine3,4 on the oxidative phosphorylation of liver and tumour mitochondria. BIBA is known as a competitive antagonist to thyroxine and structurally related compounds5. Brody6 suggests that this substance uncouples the oxidative phosphorylation in a similar way to 2,4-dinitrophenol (DNP) or barbiturates. Studying the mode of action on mitochondria BIBA was compared with amytal, which is a specific inhibitor at the flavo-protein site of the mitochondrial DPNH-cytochrome c-reductase system7. Evidence is given here that BIBA uncouples phosphorylation from respiration in isolated liver mitochondria, and we have tried to find the site of action of this uncoupling agent.

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Authors and Affiliations

  1. Department of Experimental and Clinical Chemotherapy, Robert-Rössle-Clinic, Institutes for Medicine and Biology, German Academy of Sciences, Berlin-Buch

    H. WAND & G. BACIGALUPO

Authors
  1. H. WAND
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  2. G. BACIGALUPO
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WAND, H., BACIGALUPO, G. Effects of 4-Benzyloxy-3,5-diiodo-benzoic Acid on the Oxidative Phosphorylation of Isolated Mitochondria. Nature 202, 295–296 (1964). https://doi.org/10.1038/202295a0

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