Skip to main content
SpringerLink
Log in

A Quantitative Assay for the Number of Murine Lymphoma Cells capable of Proliferation in vivo

  • Letter
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

Till, McCulloch and Becker1,2 have demonstrated that normal mouse hæmatopoietic tissue contains a class of cells which are characterized by their ability individually to give rise to macroscopic colonies in the spleens of heavily irradiated recipient mice. The ease with which the colonies may be enumerated has permitted quantitative investigations of the effects of irradiation3,4 transplantation5, and erythropoiesis6 on the number of cells with colony-forming potentiality. These varied applications suggested to us that the spleen-colony technique might be fruitfully applied to the examination of malignant tissue. The results show that a quantitative assay of the number of murine lymphoma cells capable of proliferating in vivo is, indeed, possible.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Till, J. E., and McCulloch, E. A., Rad. Res., 14, 213 (1961).

    Article  CAS  Google Scholar 

  2. Becker, A. J., McTulloch, E. A., and Till, J. E., Nature, 197, 452 (1963).

    Article  ADS  CAS  Google Scholar 

  3. Till, J. E., and McCulloch, E. A., Rad. Res., 18, 96 (1963).

    Article  CAS  Google Scholar 

  4. Till, J. E., and McCulloch, E. A., Ann. N.Y. Acad. Sci. (in the press).

  5. McCulloch, E. A., and Till, J. E., J. Cell. Comp. Physiol. (in the press).

  6. Bruce, W. R., and McCulloch, E. A., Fed. Proc., 22, 664 (1963).

    Google Scholar 

  7. Parker, R. C., Methods of Tissue Culture, third ed. (New York, Hoebe, 1961).

    Google Scholar 

  8. Puck, T. T., Rev. Mod. Phys., 31, 433 (1959).

    Article  ADS  CAS  Google Scholar 

  9. Hewitt, H. B., and Wilson, C. W., Brit. J. Cancer, 13, 69 (1959).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Medical Biophysics, University of Toronto and Ontario Cancer Institute, Toronto

    W. R. BRUCE & H. VAN DER GAAG

Authors
  1. W. R. BRUCE
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. VAN DER GAAG
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

BRUCE, W., VAN DER GAAG, H. A Quantitative Assay for the Number of Murine Lymphoma Cells capable of Proliferation in vivo. Nature 199, 79–80 (1963). https://doi.org/10.1038/199079a0

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/199079a0

  • Springer Nature Limited

This article is cited by

Search

Navigation