Skip to main content
SpringerLink
Log in

Influence of pH and Urea on the Ultra-violet Fluorescence of Several Globular Proteins

  • Letter
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

RECENT work by Weber1–3 has shown that the ultraviolet fluorescent spectra of globular proteins differ from those predicted for the summation of the independent contributions of their aromatic amino-acid constituents. Those examples investigated which contained tyrosine, but no tryptophan, showed a tyrosine emission spectrum, but with a reduced quantum yield. Those containing both tyrosine and tryptophan showed an emission spectrum predominantly resembling that of tryptophan alone, with an emission maximum in the range 320–350 mµ. The present communication will be concerned solely with proteins of the second category.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Weber, G., Biochem. J., 75, 335 (1960).

    Article  CAS  Google Scholar 

  2. Teale, F., and Weber, G., Biochem. J., 65, 476 (1957).

    Article  CAS  Google Scholar 

  3. Weber, G., Biochem. J., 75, 345 (1960).

    Article  CAS  Google Scholar 

  4. Weber, G., and Teale, F., Disc. Farad. Soc., 27, 134 (1959).

    Article  Google Scholar 

  5. Forster, T., Fluoreszenz Organischen Verbindugen (Vanderhoeck and Ruprecht, Gottingen, 1951).

    Google Scholar 

  6. Yang, J., and Foster, J., J. Amer. Chem. Soc., 76, 1588 (1954).

    Article  CAS  Google Scholar 

  7. Williams, E., and Foster, J., J. Amer. Chem. Soc., 82, 242 (1960).

    Article  CAS  Google Scholar 

  8. Katz, S., and Klotz, I., Arch. Biochem. Biophys., 44, 351 (1953).

    Article  CAS  Google Scholar 

  9. Edelhoch, H., J. Amer. Chem. Soc., 79, 6100 (1957).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Naval Medical Research Institute, National Naval Medical Center,

    R. F. STEINER

  2. National Institute of Arthritis and Metabolic Disease, National Institutes of Health, Bethesda, Maryland

    H. EDELHOCH

Authors
  1. R. F. STEINER
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. EDELHOCH
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

STEINER, R., EDELHOCH, H. Influence of pH and Urea on the Ultra-violet Fluorescence of Several Globular Proteins. Nature 192, 873–874 (1961). https://doi.org/10.1038/192873a0

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/192873a0

  • Springer Nature Limited

Search

Navigation