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A Study of the Temperature Dependence of Tryptophan Fluorescence Lifetime in the Range of –170 to +20°С in Various Solvents

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Abstract

The temperature dependences (–170...+20°C) of the fluorescence lifetime of tryptophan molecules in an aqueous solution, in solutions of glycerol (50 and 75% by volume), dimethyl sulfoxide (50 and 75% by volume) and in 1 M aqueous solution of trehalose were studied. In this temperature range, the fluorescence kinetics at room temperature in all samples were best approximated by three exponents with characteristic times of τ1 ~ 3 ns, τ2 ~ 4 ns, and τ3 ~ 15 ns. The temperature dependences of the fluorescence lifetime of the fastest and medium components in the temperature range from –60 to 10°C were found to be directed in opposite directions (in antiphase). To explain these temperature dependences, a new model of the nature of multicomponent fluorescence was proposed, which considered the transition of the tryptophan molecule from the excited state to the charge-transfer state, the reverse transition to the excited state, as well as the radiative and nonradiative transitions of the charge-transfer state to the ground state. The results we obtained can serve as a basis for the interpretation of the experimental dependences of the lifetime of tryptophan fluorescence on the temperature in proteins.

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ACKNOWLEDGMENTS

The authors are grateful to E. P. Lukashev for his help in the measurements of the absorption and fluorescence spectra of tryptophan in an aqueous solution.

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Authors and Affiliations

  1. Department of Biology, Moscow State University, 119234, Moscow, Russia

    V. Z. Paschenko, V. V. Gorokhov, B. N. Korvatovsky, P. P. Knox, N. P. Grishanova & S. N. Goryachev

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  1. V. Z. Paschenko
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  2. V. V. Gorokhov
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  3. B. N. Korvatovsky
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  4. P. P. Knox
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  5. N. P. Grishanova
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  6. S. N. Goryachev
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Corresponding author

Correspondence to V. Z. Paschenko.

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Translated by E. Puchkov

Abbreviations: Trp—tryptophan; CTS—charge-transfer state; DMSO—dimethylsulfoxide; HB—hydrogen bonds.

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Paschenko, V.Z., Gorokhov, V.V., Korvatovsky, B.N. et al. A Study of the Temperature Dependence of Tryptophan Fluorescence Lifetime in the Range of –170 to +20°С in Various Solvents. BIOPHYSICS 66, 385–394 (2021). https://doi.org/10.1134/S0006350921030143

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  • DOI: https://doi.org/10.1134/S0006350921030143

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