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Influence of Oxygen Concentration and Mechanical Factors on Differentiation of Connective Tissues in vitro

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Abstract

MARCHAND1 postulated that osteoblasts may differentiate from mesenchymal cells. Recently, it was observed that a strain of cells, derived from adult skeletal muscle, elaborated in tissue culture a sub-stance that appeared to be chondro-osteoid2. Since the factors responsible for this cell behaviour were unknown, investigation of the effects of varying mechanical and nutritional factors on a reproducible in vitro system of osteogenesis was made. Hanging-drop, Maximov double cover-slip cultures were established from 20-day-old chick embryo tibial cortex and were maintained as described by Fell3.

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References

  1. Marchand, F., “Der Prozess der Wundheilung”, in Deutsche Chirurgie, 16, 1 (F. Enke, Stuttgart, 1901).

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  2. Bassett, C. A. L., J. Bone and Joint Surg., 41, A, 773 (1959).

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  3. Fell, H. B., J. Anat., 66, 157 (1932).

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  6. Maximov, A. A., in von Mollendorff, M., Handbuch der mikroskopischen Anatomie des Menschen, 2, Part 1, 232 (J. Springer, Berlin, 1927).

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Authors and Affiliations

  1. Orthopaedic Research Laboratories, Columbia University, College of Physicians and Surgeons, New York

    C. ANDREW L. BASSETT & INGEBORG HERRMANN

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  1. C. ANDREW L. BASSETT
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  2. INGEBORG HERRMANN
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BASSETT, C., HERRMANN, I. Influence of Oxygen Concentration and Mechanical Factors on Differentiation of Connective Tissues in vitro . Nature 190, 460–461 (1961). https://doi.org/10.1038/190460a0

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