Abstract
MANY mobile phases are found in the paper chromatography of sugars; but the separation of sucrose, fructose and glucose is somewhat difficult, because their R F values are very near each other1. The separation is enough for identification purposes, but it is not good enough for elution or photometric readings, because the sugars are still partly mixed. The solvent suggested below divides the sugars quite well with the following R F values (at 20° C.): sucrose, 0.17; glucose, 0.26; mannose, 0.30; arabinose, 0.32; fructose, 0.32; xylose, 0.33; ribose, 0.41.
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References
Lederer, M., and Lederer, E., Chromatography, 158 (Elsevier Pub. Co., 1953).
Giovannozzi-Sermanni, G., I° Congrès International Scientifique du Tabac., Paris, 7–14 Septembre, 1955.
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GIOVANNOZZI-SERMANNI, G. A New Solvent for Quantitative Paper Chromatography of Sugars. Nature 177, 586–587 (1956). https://doi.org/10.1038/177586b0
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DOI: https://doi.org/10.1038/177586b0
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