Abstract
IN the course of an investigation1 of rat liver, with respect to hormonal effects on the yield and composition of fractions isolated by differential centrifugation in 0.25 M sucrose medium2, determinations have been made of the particle number in freshly isolated ‘large-particle’ (‘mitochondrial’) fractions. These fractions were obtained from liver homogenates, prepared with a Potter-type homogenizer and centrifuged at a low speed to remove ‘debris’ containing the nuclei, by farther centrifugation for 20 min. in an ‘Angle 13’ (Measuring and Scientific Equipment, Ltd.) centrifuge at 12,000 g (maximum value, at extreme tip of tube). The particles were suspended in 1.8 M sucrose solution and counted under dark-ground illumination as described by Allard et al. 3. Under these conditions (in 1.8 M sucrose) the particle size-range is decreased (compressed); all visible particles up to and including the mitochondrial size-range were counted.
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References
Reid, E., (a) Biochem. J., 58, xxiii (1954); (b) Proc. Symp. on “The Hypophyseal Growth Hormone” (Henry Ford Hospital, Detroit) (to be published); (c) Nature, [175, 461 (1955 )].
Schneider, W. C., J. Biol. Chem., 176, 259 (1948).
Allard, C., Mathieu, R., de Lamirande, G., and Cantero, A., Cancer Research, 12, 407 (1952).
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GREENBAUM, A., SLATER, T. & REID, E. Effect of Growth Hormone on the Large-Particle Content of Rat Liver. Nature 175, 506–507 (1955). https://doi.org/10.1038/175506b0
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DOI: https://doi.org/10.1038/175506b0
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