Abstract
ON February 23, 1927, five small fragments of a mouse adenocarcinoma (Frankfurt strain of Ehrlich) were cultivated as hanging drop cultures1. With slight modifications, the culture medium used has remained practically the same throughout this period of twelve years. The medium used for cover-glass cultures consisted of one volume of a plasma mixture (equal parts of chick and rat plasma) and one volume of embryonic tissue juice of chick. For cultures in Carrel flasks, the solid medium was composed of equal parts of dilute chick and rat plasma coagulated by a drop of embryonic tissue juice; the supernatant liquid phase, 0.5 c.c., consists of 20 per cent embryonic tissue juice (chick), 20 per cent rat serum and 60 per cent ‘Tyrodc’ solution.
Similar content being viewed by others
References
Z. Krebsforsch., 25, 89 (1927).
Fischer, A., "Gewebezüchtung" (Munich, 1930).
Ann. Anat. path., 18, 665 (1931).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
FISCHER, A., DAVIDSOHN, F. Present Condition of a Twelve-Year Old Pure Strain of Carcinoma Cells in vitro. Nature 143, 436–437 (1939). https://doi.org/10.1038/143436b0
Issue Date:
DOI: https://doi.org/10.1038/143436b0
- Springer Nature Limited
We’re sorry, something doesn't seem to be working properly.
Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.