Abstract
Anin vitro trial with carbendazim fungicide on the growth profile of the predatory fungusDuddingtonia flagrans was undertaken andin vivo trials in sheep and buffaloes, fed on chlamydospores ofD. flagrans and administered albendazole anthelmintic, were conducted. Although no growth inhibition was detected at a carbendazim concentration of 0.05 ppm, growth inhibition was recorded of 50% and above at concentrations of 0.25 and 1.00 ppm (p<0.001) and of around 90% at concentrations of 2.00 to 5.00 ppm (p<0.0001). Scanty recovery of the fungus was made from faecal culture 48 h following a single dose of albendazole both in sheep and buffaloes. However, profuse fungal recovery was made from 96 h post dosing onwards. When the drug was used as an intraruminal slow-release capsule, no faecal fungal recovery could be made from day 3 after administration of the capsule, when the albendazole sulphoxide concentration was around 1.0 μg/ml. However, profuse and scanty fungal recovery could be made on days 1 and 2, respectively, after administration of the capsule, when the plasma albendazole sulphoxide concentration was around 0.4 and 0.9 μg/ml, respectively. The implications for use of a combination of anthelmintics and biological control in sustainable parasite control programmes are discussed.
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REFERENCES
Githigia, S.M., Thamsborg, S.M., Larsen, M., Kyvsgaard, N.C. and Nansen, P., 1997. The preventive effect of the fungus Duddingtonia flagrans on trichostrongyle infections of lambs on pasture. International Journal for Parasitology, 27, 931-939
Gronvold, J., Wolstrup, J., Larsen, M., Henriksen, S.A. and Nansen, P., 1993. Biological control of Ostertagia ostertagi by feeding selected nematode-trapping fungi to calves. Journal of Helminthology, 67, 31-36
Horsfall, J.G., 1956. Principles of Fungicidal Action, (Chronica Botanica Co., Waltham, MA), 279
Lalithakumari, D., Mrinalini, C., Chandra, A.B. and Anamalai, P., 1996. Strain improvement by protoplast fusion for enhancement of biocontrol potential integrated with fungicide tolerance in Trichoderma spp. Journal of Plant Diseases and Protection, 103, 206-212
Larsen, M., 2002. Biological control in a global perspective — a review with emphasis on Duddingtonia flagrans. In: Biological Control of Nematode Parasites of Small Ruminants in Asia, (FAO Animal Production and Health Paper; FAO Technical Cooperation Project in Malaysia, FAO, Rome), 19-37
Larsen, M., Nansen, P., Grondahl, C., Thamsborg, S.M., Gronvold, J., Wolstrup, J., Henriksen, S.A. and Monrad, J., 1996. The capacity of the fungus Duddingtonia flagrans to prevent strongyle infection in foals on pasture. Parasitology, 133, 1-6
Larsen, M., Faedo, M., Waller, P.J. and Hennessy, D.R., 1998. The potential of nematophagous fungi to control the free-living stages of nematode parasites of sheep: studies with Duddingtonia flagrans. Veterinary Parasitology, 76, 121-128
Nansen, P., Larsen, M., Roepstorff, A., Gronvold, J., Wolstrup, J. and Henriksen, S.A., 1996. Control of Oesophagostomum dentatum and Hyostrongylus rubidus in outdoor reared pigs by daily feeding with the microfungus Duddingtonia flagrans. Parasitology Research, 82, 580-584
Nari, A. and Eddi, C., 2002. F.A.O. Perspectives and goals. In: Biological Control of Nematode Parasites of Small Ruminants in Asia, (FAO Animal Production and Health Paper; FAO Technical Cooperation Project in Malaysia, FAO, Rome), 1-4
Papavizas, G C., Lewis, J.A. and Abd-moity, T.H., 1982. Evaluation of new biotypes of Trichoderma harzianum for tolerance to benomoyl and enhanced biocontrol capabilities. Phytopathology, 72, 126-132
Rubner, A., 1996. Revision of predacious hypomycetes in the Dactylaria—Monacrosporium complex. Studies in Mycology, No. 39, (Utrecht, Centraalbureau voor Schimmelcultures), 134
Sanyal, P.K., 1994. Pharmacokinetic behaviour of fenbendazole in buffalo and cattle. Journal of Veterinary Pharmacology and Therapeutics, 17, 1-4
Sanyal, P.K., 1995. Kinetic disposition of triclabendazole in buffalo compared to cattle. Journal of Veterinary Pharmacology and Therapeutics, 18, 370-374
Sanyal, P.K., 1997. Disposition kinetics of albendazole in buffalo and cattle. Journal of Veterinary Pharmacology and Therapeutics, 20, 240-242
Sanyal, P.K., 1998. Integrated parasite management in ruminants in India: a concept note. In: Biological Control of Gastro-intestinal Parasites of Ruminants using Predacious Fungi, (FAO Animal Production and Health Paper 141; FAO, Rome), 54-65
Sanyal, P.K., 2000. Screening for Indian isolates of predacious fungi for use in biological control against nematode parasites of ruminants. Veterinary Research Communications, 24, 55-62
Sanyal, P.K., 2002. Worm control in ruminants in India: prospects of biological control for integrated nematode parasite management. In: Biological Control of Nematode Parasites of Small Ruminants in Asia, (FAO Animal Production and Health Paper; FAO Technical Cooperation Project in Malaysia, FAO, Rome), 76-85
Sanyal, P.K. and Singh, D.K., 1995. Proceedings of Workshop on Control Strategy Against Gastrointestinal Parasites in Dairy Animals Using Medicated Urea Molasses Blocks, (National Dairy Development Board, Anand, India)
Sanyal, P.K. and Mukhopadhyaya, P.N., 2003a. Top dressing of feed with desiccated chlamydospores of Duddingtonia flagrans for biological control of pre-parasitic stages of ovine Haemonchus contortus. Veterinary Research Communications, 27, 381-390
Sanyal, P.K. and Mukhopadhyaya, P.N., 2003b. Biological control of nematode parasites of buffaloes and cattle using dried chlamydospores of Duddingtonia flagrans as feed supplement. Buffalo Journal (in press).
Theodorides, V.J., Gyurik, R.J., Kingsbury, W.D. and Parish, R.C., 1976. Anthelmintic activity of albendazole against liver flukes, tapeworms, lung and gastrointestinal round worms. Experientia, 32, 702-703
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Sanyal, P., Chauhan, J. & Mukhopadhyaya, P. Implications of Fungicidal Effects of Benzimidazole Compounds on Duddingtonia flagrans in Integrated Nematode Parasite Management in Livestock. Vet Res Commun 28, 375–385 (2004). https://doi.org/10.1023/B:VERC.0000034997.50332.77
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DOI: https://doi.org/10.1023/B:VERC.0000034997.50332.77