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The effects of unilateral testicular ischemia and hemicastration on contralateral testicular IGF-1 level, histology and lipid peroxidation

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Abstract

Hemicastration is followed by compansatoryhypertrophy whereas unilateral testiculartorsion is followed by atrophy in contralateraltesticle in rats. Insulin-likegrowth factor (IGF-1) has important roles intesticular paracrine and autocrine functions. Inthis study it was aimed to compare ischemicparameters and IGF-1levels in the contralateral testicle inunilateral spermatic cord ligation, testiculartorsion, and hemicastratron. 32 wistar ratswere equally altocated into sham,ligation, torsion, and hemicastration groups.In ligation group, right spermatic cord wasligated with 3/0 silk suture. In thetorsion group, right testis was tcrsed for720 degrees. In hemicastration group, rightorchiectomy was done. 48 hours later leftorchiectomy was done in all groups.Malondialdehyde (MDA) and IGF-1 levels weredetermined in the testicle. Average values ofthe groups werecompared with Anova followed by Dunnett T3multiple comparison tests. MDAlevels were significantly reduced in ligationand torsion groups (p < 0.05). This reductionwas more prominent in hemicastration group(p < 0.05). Contralateral testicular IGF-1levels in ligation and torsion groups were notdifferent compared with the sham group. Lefttesticular IGF-1 level in the hemicastrationgroup was decreased significantly comparedwith other groups (p < 0.05). Histological.changes evaluated. Contralateral Johnsen'stesticular biopsy scores were significantlydecreased in all experimental groups but meantubular diameter was not changed in all groups.

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Authors and Affiliations

  1. Department of Pediatric Surgery, Suleyman Demirel University, Medical School, Turkey

    Cagri Savas, I. Faruk Ozguner, Meltem Ozguner & Namik Delibas

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  1. Cagri Savas
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  2. I. Faruk Ozguner
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  3. Meltem Ozguner
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  4. Namik Delibas
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Correspondence to Cagri Savas.

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Savas, C., Ozguner, I.F., Ozguner, M. et al. The effects of unilateral testicular ischemia and hemicastration on contralateral testicular IGF-1 level, histology and lipid peroxidation. Int Urol Nephrol 35, 231–235 (2003). https://doi.org/10.1023/B:UROL.0000020287.21049.2d

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