Abstract
Transformed roots of Lupinus mutabilis cv. Potosi induced by Agrobacterium rhizogenes strain R1601 were cultured on Murashige and Skoog-based medium lacking kanamycin sulphate, or with this antibiotic at 40 mg l−1. The neomycin phosphotransferase gene in the genome of transformed roots was confirmed by non-radioactive Southern hybridisation. Neomycin phosphotransferase protein was detected by ELISA. Transformed roots synthesised isoflavones, but not quinolizidine alkaloids; the latter are typical secondary metabolites of lupin normally produced in aerial parts of the plant. Genistein and 2′-hydroxygenistein, were the main secondary metabolites in cultured, transformed roots, whereas the glycoside genistin was more abundant in roots of non-transformed plants. Wighteone concentrations in transgenic roots were higher than those of non-transformed roots. Transformed roots produced twice the concentration of isoflavones compared with roots from non-transformed plants, indicating that Ri plasmid T-DNA genes modified isoflavone concentration and pattern of biosynthesis.
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Babaoglu, M., Davey, M.R., Power, J.B. et al. Transformed Roots of Lupinus mutabilis: Induction, Culture and Isoflavone Biosynthesis. Plant Cell, Tissue and Organ Culture 78, 29–36 (2004). https://doi.org/10.1023/B:TICU.0000020386.03780.ea
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DOI: https://doi.org/10.1023/B:TICU.0000020386.03780.ea