Abstract
Ranunculus asiaticus is an important ornamental species mainly cultivated in the countries surrounding the Mediterranean sea. So far the multiplication of this plant has been mainly carried out by seed and rhizome division; however, these systems present many drawbacks. Tissue culture is an attractive alternative for accelerated propagation of selected and indexed genotypes. In this paper we review the work carried out on in vitro culture of R. asiaticus and present a flow chart for its commercial production, using axillary budding and embryogenesis. Although the price of micropropagated plants is higher compared to traditional material (seedlings and rhizomes from seed populations), it should be considered that tissue cultured plants have a better rhizome yield per plant; moreover, the tissue culture approach allows to offer clonal material of selected lines.
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References
Beruto M (1997) Agar and gel characteristics with special reference to micropropagation systems of Ranunculus asiaticu L. PhD Thesis, Gent, Faculteit Landbouwkundige en Toegepaste Biologische Wetenschappen, University Gent, Belgium, pp. 21–44, 125-184
Beruto M & Debergh P (1992) Somatic embryogenesis in Ranunculus asiaticus L. hybr. thalamus cultivated in vitro. Plant Cell Tiss. Org. Cult. 29: 161–165
Beruto M & Portogallo C (2000) Factors affecting the growth of Ranunculus in vitro. Acta Hort. 520: 163–170
Beruto M, Siccardi G & Lercari G (1989) Risultati preliminari nella propagazione in vitro del ranuncolo. In: Giaggioli G (ed) Proceedings of Symposium on 'Colture in vitro emicropropagazione in ortoflorofrutticoltura' Abstracts (p. 33). Cesena, Italy. Lo Scarabeo Press, Bologna
Beruto M, Cane G & Debergh P (1996a) Field performance of tissue-cultured plants of Ranunculus asiaticus L. Sci. Hortic. 66: 229–239
Beruto M, Curir P & Debergh P (1996b) Callus growth and somatic embryogenesis in thalamus tissue of Ranunculus asiaticus L. cultivated in vitro: cytokinin effect and phenol metabolism. In Vitro Cell. Dev. Biol. Plant 32: 154–160
Beruto M, Curir P & Debergh P (1999) Influence of agar on in vitro cultures: II. Biological performance of Ranunculus on media solidified with three different agar brands. In Vitro Cell. Dev. Biol. Plant 35: 94–101
Beruto M, La Rosa C & Portogallo C (2001) Effects of agar impurities on in vitro propagation of Ranunculus asiaticus L. Acta Hort. 560: 399–402
De Proft M, Maene L & Debergh P (1985) Carbon dioxide and ethylene evolution in the culture atmosphere of Magnolia cultured in vitro. Physiol. Plant 65: 375–379
Lercari G, Littardi M & Garibaldi E (1985) In vitro propagation of Ranunculus: experimental results. Riv. Ortoflorofrutt. It. 69: 321–326
Maia E, Bettachini B, Beck D & Marais A (1973) Régénération de renoncules par culture d'apex in vitro. Ann. Phytopathol. 5: 125–129
Meynet J (1985) Multiplication et sélection de la renoncule. Pépinièristes Horticultéurs Maraichers Revue Horticole, 254: 55–62
Meynet J (1993) Ranunculus. In: De Hertogh A & Le Nard M (eds) The Physiology of Flower Bulbs (pp. 603–610). Elsevier Science Publishers, The Netherlands
Meynet J & Duclos A (1990a) Culture in vitro de la renoncule des fleuristes (Ranunculus asiaticus L.). I. Néoformation et multiplication végétative in vitro de plantes à partir de tron?on de thalamus. Agronomie 2: 157–162
Meynet J & Duclos A (1990b) Culture in vitro de la renoncule des fleuristes (Ranunculus asiaticus L.). II. Production de plantes par culture d'anthères in vitro. Agronomie 10: 213–218
Meynet J & Duclos A (1990c) Culture in vitro de la renoncule des fleuristes (Ranunculus asiaticus L.). III. Etude des plantes produites par embryogenèse somatique à partir des tissus superficiels de l'anthère. Agronomie 10: 285–290
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15: 473–497
Pugliesi C, Rabaglio M, Cecconi F & Baroncelli S (1992) Plant regeneration from tissue cultures of Persian buttercup (Ranunculus asiaticus L.). Plant Cell Tiss. Org. Cult. 28: 125–128
Statistisch Jaarboek (2001) Vereniging van Bloemenveilingen in Nederland (VBN), Leiden, Holland (p. 117)
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Beruto, M., Debergh, P. Micropropagation of Ranunculus Asiaticus: A Review and Perspectives. Plant Cell, Tissue and Organ Culture 77, 221–230 (2004). https://doi.org/10.1023/B:TICU.0000018416.38569.7b
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DOI: https://doi.org/10.1023/B:TICU.0000018416.38569.7b