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Comparison of Anther and Microspore Culture in the Embryogenesis and Regeneration of Rye (Secale cereale)

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Abstract

Anther culture in solid and liquid medium and isolated microspore culture were compared in rye genotypes with potential agronomic characteristics. Some important factors influencing androgenic capacity were optimised. Three weeks cold pre-treatment of spikes and two days mannitol pre-treatment of anthers maximized callus and green plant yield in both culture methods. Intensity order of the culture methods in callus and green plant production was: isolated microspore culture, anther culture in liquid medium and anther culture in solid medium. Genotype ability of embryogenesis followed the same pattern in both cultivation methods. Kinetin (BA) with genotype dependent concentrations created the most effective regeneration conditions.

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Authors and Affiliations

  1. Laboratory of Plant Physiology and Molecular Biology, Department of Biology, University of Turku, FIN-20014, Turku, Finland

    Rui Ma, Yang-Dong Guo & Seppo Pulli

Authors
  1. Rui Ma
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  2. Yang-Dong Guo
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  3. Seppo Pulli
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Correspondence to Rui Ma.

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Ma, R., Guo, YD. & Pulli, S. Comparison of Anther and Microspore Culture in the Embryogenesis and Regeneration of Rye (Secale cereale). Plant Cell, Tissue and Organ Culture 76, 147–157 (2004). https://doi.org/10.1023/B:TICU.0000007294.68389.ed

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