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Differentiation of the Dolly Varden Char (Salvelinus malma) and the Taranetz Char (Salvelinus taranetzi) Inferred from the PCR–RFLP Analysis of Mitochondrial DNA

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Abstract

Genetic differentiation of two sympatric char species, the Dolly Varden char (Salvelinus malma Walbaum) and the Taranetz char (S. taranetzi Kaganovski), has been studied. Restriction fragment length polymorphism (RFLP) analysis has been used to compare three mitochondrial DNA (mtDNA) fragments (ND1/ND2, ND5/ND6, and Cytb/D-loop) amplified via polymerase chain reaction (PCR). The divergence between S. malma and S. taranetzi inferred from mtDNA nucleotide sequences is 2.8%; between S. leucomaenis and S. taranetzi, 7.1%; and between S. malma and S. leucomaenis, 7.5%. The absence of common haplotypes and the degree of divergence indicate that the Dolly Varden char and the Taranetz char are genetically isolated and confirm that S. taranetzimay be regarded as a separate species.

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Authors and Affiliations

  1. Institute of Marine Biology, Russian Academy of Sciences, Vladivostok, 690041, Russia

    A. G. Oleinik, L. A. Skurikhina & Vl. A. Brykov

  2. Far East State University, Vladivostok, 690000, Russia

    Vl. A. Brykov

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  1. A. G. Oleinik
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  2. L. A. Skurikhina
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  3. Vl. A. Brykov
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Oleinik, A.G., Skurikhina, L.A. & Brykov, V.A. Differentiation of the Dolly Varden Char (Salvelinus malma) and the Taranetz Char (Salvelinus taranetzi) Inferred from the PCR–RFLP Analysis of Mitochondrial DNA. Russian Journal of Genetics 40, 300–306 (2004). https://doi.org/10.1023/B:RUGE.0000021631.11738.25

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