Abstract
We studied the influence of combined microinjection of a gene engineering construct and site-specific endonuclease SalIin the pronucleus on preimplantation development of (CBA × C57BL)F1 mouse embryos in vitro. The rate of survival of the embryos was estimated according to their capacity to develop until the blastocyst stage and hatch from zona pellucida. The results obtained suggest that the microinjection of exogenous DNA jointly with endonuclease SalI at concentrations from 0.1 to 0.01 U/μl decreased reliably the rate of survival, as compared to the control (p < 0.05 and p < 0.01, respectively). However, a decrease of endonuclease SalI concentration in the injection mixture to 0.01 U/μl enhanced the capacity of mouse embryos to develop until the blastocyst stage and hatch from zona pellucida, as compared to the embryos microinjected with exogenous DNA and endonuclease SalI at a higher concentration.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
Aguerre, A. and Castro-Palomino, N., Expression of Human Erythropoetin Transgene and of the Endogenous WAP Gene in the Mammary Gland of Transgenic Rabbit during Gesta-tion and Lactation, Transgen. Res., 1998, vol. 7, pp. 31–317.
Bishop, J.O. and Smith, P., Mechanism of Chromosomal Integration of Microinjected DNA, Mol. Biol. Med., 1989, vol. 6, pp. 283–298.
Brenneman, M., Gimble, F.S., and Wilson, J.H., Stimulation for Intrachromosomal Homologous Recombination in Human Cells by Electroporation with Site-Specific Endonu-clease, Proc. Natl. Acad. Sci. USA, 1996, vol. 85, pp. 83?–840.
Brinster, R.L., Chen, H.Y., Traumbauer, M.E., et al., Factors Affecting the Efficiency of Introducing Foreign DNA Into Mice by Microinjection, Proc. Natl. Acad. Sci. USA, 1985, vol. 82, pp. 4438–4442.
Krimpenfort, P., Rademakers, A., Eyestone, W., et al., Gen-eration of Transgenic Dairy Cattle Using in vitro Embryo Production, Biotechnol., 1991, vol. 9, pp. 844–847.
Persuy, M.A., Legrain, S., Printz, C., et al., High-Level, Stage-and Mammary Tissue-Specific Expression of a Caprine β-Casein-Encoding Minigene Driven by a β-Casein Promoter in Transgenic Mice, Gene, 1995, vol. 165, p. 291.
Popova, E.A., Krivokharchenko, A.S., and Vil'yanovich, L.I., A Study of the Effects of Various Solutions Used for Microinjection of gene Engineering Constructs on Preim-plantation Development of Mouse Embryos, Ontogenez, 2002, vol. 33, no. 2, pp. 139–141.
Seo, B.B., Kim, C.H., Yamanouchi, K., et al., Co-Injection of Restriction Enzyme with Foreign DNA Into the Pronucleus for Elevating Production Efficiencies of Transgenic Animals, Animal Reprod. Sci., 2000, vol. 63, pp. 113–122.
Ward, J.F., DNA Damage Produced by Ionizing Radiation in Mammalian Cells: Identities, Mechanisms of Formation, and Reparability, Prog. Nucl. Acid. Res. Mol. Biol., 1988, vol. 35, pp. 95–125.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Shishimorova, M.S., Ivanova, L.B. & Ryabykh, V.P. Influence of Combined Microinjection of Gene Engineering Construct and Endonuclease on Preimplantation Development of Mouse Embryos in vitro . Russian Journal of Developmental Biology 35, 316–318 (2004). https://doi.org/10.1023/B:RUDO.0000043826.95029.1e
Issue Date:
DOI: https://doi.org/10.1023/B:RUDO.0000043826.95029.1e