Abstract
Immunocytolocalization experiments indicate that carbonic anhydrase, phosphoribulokinase, and P-glycerate kinase are near neighbors of Rubisco in the pea leaf chloroplast stroma. Direct transfer of ribulose bisphosphate and gaseous CO2 from phosphoribulokinase and carbonic anhydrase to Rubisco, and direct transfer of P-glycerate from Rubisco to P-glycerate kinase in the chloroplast stroma, is then a possibility. Rubisco activase, responsible for the removal of inhibitory sugar phosphates that bind to the active site of Rubisco in the dark, also appears to be co-localized with Rubisco.
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Anderson LE, Goldhaber-Gordon IM, Li D, Tang X-Y, Xiang M and Prakash N (1995a) Enzyme-enzyme interaction in the chloroplast: glyceraldehyde-3-phosphate dehydrogenase, triose phosphate isomerase and aldolase. Planta 196: 245–255
Anderson LE, Wang X and Gibbons JT (1995b) Three enzymes of carbon metabolism, or their antigenic analogs, in pea nuclei. Plant Physiol 108: 659–667
Anderson LE, Gibbons JT and Wang X (1996a) Distribution of ten enzymes of carbon metabolism in pea (Pisum sativum ) chloroplasts. Int J Plant Sci 157: 525–538
Anderson LE, Tang X-Y, Johansson G, Wang X, Marques IA and Macioszek J (1996b) Interaction between chloroplast phosphoglycerate kinase and glyceraldehyde-3-phosphate dehydrogenase. Adv Mol Cell Biol 15A 273–279
Anderson JB, Carol AA, Brown VK and Anderson LE (2003) A quantitative method for assessing co-localization in immuno-labeled thin section electron micrographs. J Struct Biol 143: 95–106
Anderson LE, Ringenberg MR and Carol AA (2004) Cytosolic glyceraldehyde-3-P dehydrogenase and the B subunit of the chloroplast enzyme are present in the pea leaf nucleus. Protoplasma 223: 33–43
Babadzhanova MP, Babadzhanova MA and Aliev KA (2002) Free and membrane-bound multienzyme complexes with Calvin cycle activities in cotton leaves. Rus J Plant Physiol 49: 592–597
Badger MR and GD Price (2003) CO2 concentrating mechanisms in cyanobacteria: molecular components, their diversity and evolution. J Exp Bot 54: 609–622
Büchen-Osmond C, Portis Jr AR and Andrews TJ (1992) Rubisco activase modi es the appearance of Rubisco in the electron microscope. In: Murata N (ed) Research in Photosynthesis, IX International Congress on Photosynthesis, Vol 3, pp 653–656. Kluwer Academic Publishers, Dordrecht, The Netherlands
Gontero B, Cárdenas ML and Ricard J (1988) A functional ve-enzyme complex of chloroplasts involved in the Calvin cycle. Eur J Biochem 173: 437–443
Henze K, Schnarrenberger C, Kellermann J and Martin W (1994) Chloroplast and cytosolic triosephosphate isomerases from spinach: purification, microsequencing and cDNA cloning of the chloroplast enzyme. Plant Mol Biol 26: 1961–1973
Jebanathirajah JA and Coleman JR (1998) Association of carbonic anhydrase with a Calvin cycle enzyme complex in Nicotiana tabacum. Planta 204: 177–182
Kachru RB and Anderson LE (1974) Chloroplast and cytoplasmic enzymes V Pea leaf carbonic anhydrases. Planta 118: 235–240
Macioszek J, Anderson JB and Anderson LE (1990) Isolation of chloroplastic phosphoglycerate kinase Kinetics of the two enzyme phosphoglycerate kinase/glyceraldehyde-3-phosphate dehydrogenase couple. Plant Physiol 94: 291–296
Marques IA, Ford DM, Muschinek G and Anderson LE (1987) Photosynthetic carbon metabolism in isolated pea chloroplasts: metabolite levels and enzyme activities. Arch Biochem Biophys 252: 458–466
Portis Jr AR (2003) Rubisco activase–Rubisco 's catalytic chaperone. Photosynth Res 75: 11–27
Sainis JK and GC Harris (1986) The association of ribulose-1, 5-bisphosphate carboxylase with phosphoriboisomerase and phosphoribulokinase. Biochem Biophys Res Commun 139: 947–954
Schmidt M, Svendsen I and Feierabend J (1995) Analysis of the primary structure of the chloroplast isozyme of triosephosphate isomerase from rye leaves by protein and cDNA sequencing indicates a eukaryotic origin of its gene. Biochim Biophys Acta 1261: 257–264
Süss KH, Arkona C, Manteuffel R and Adler K (1993) Calvin cycle multienzyme complexes are bound to chloroplast thylakoid membranes of higher plants in situ. Proc Natl Acad Sci 90: 5514–5518
Wang X, Tang X-Y and Anderson LE (1996) Enzyme-enzyme interaction in the chloroplast: physical evidence for association between phosphoglycerate kinase and glyceraldehyde-3-phosphate dehydrogenase in vitro. Plant Sci 117: 45–53
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Anderson, L.E., Carol, A.A. Enzyme Co-Localization with Rubisco in Pea Leaf Chloroplasts. Photosynthesis Research 82, 49–58 (2004). https://doi.org/10.1023/B:PRES.0000040443.92346.37
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DOI: https://doi.org/10.1023/B:PRES.0000040443.92346.37