Summary
Chloroplastic and cytoplasmic forms of pea (Pisum sativum L.) leaf carbonic anhydrase were separated by isoelectric focusing. The two forms have identical pH optima, 7.0 for the hydration reaction and 7.5 for the dehydration reaction, and identical Michaelis constants for CO2, 0.03 M. Neither isozyme is affected by any of several compounds involved in carbon metabolism in the green plant.
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IV=Anderson and Pacold (1972).
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Kachru, R.B., Anderson, L.E. Chloroplast and cytoplasmic enzymes. Planta 118, 235–240 (1974). https://doi.org/10.1007/BF00384779
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DOI: https://doi.org/10.1007/BF00384779