Abstract
Shooting stage roots of cereal plants varying in Al tolerance: rye (Secale L.), triticale (Triticale), barley (Hordeum L.) and four wheat (Triticum L.) varieties grown at pH 7 (controls) and stressed during 10 days at pH 4 with aluminium concentrations ranging from 0 to 40 mg dm−3 were back titrated with NaOH. Back-titration data were used for estimation of variable surface charge vs. pH dependencies and apparent surface dissociation constants distribution functions. Roots grown at pH 4 without Al addition had apparently the same charge properties as the control roots. With the increase of the concentration of the aluminium treatment, the variable surface charge of the roots decreased, beginning at 5 mg Al dm−3 for barley, at 10 mg Al dm−3 for wheat and triticale (20 mg Al dm−3 for acid resistant wheat Inia), and at 40 mg Al dm−3 for rye, and a weakening of root surface acidity was deduced from the decrease in average surface dissociation constant of the root surface. The latter was connected with an increase of the relative number of weakly acidic surface sites and a simultaneous decrease in surface groups of medium and strong acidity. During Al stress the density of variable surface charge of the roots decreased markedly. Changes in surface charge properties of Al-tolerant species were less intensive than for Al-sensitive ones.
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References
Allan D L, Shann J R and Bertsch P M 1990 Role of root cell walls in iron deficiency of soybean (Glycine max) and alu-minum toxicity of wheat (Triticum aestivum). In Plant Nutri-tion – Physiology and Applications, Ed. Van Beusichem M L. pp. 345–349. Kluwer Academic Publishers, Dordrecht.
Aniol A 1985 Aluminium tolerance in cereals. Biuletyn IHAR 156, 7–11 (in Polish).
Balsberg-Pahlsson A M 1995 Growth, radicle and root hair development of Deschampsia flexuosa (L.) Trin. seedlings in relation to soil acidity. Plant Soil 175, 125–132.
De Wit J C M, Van Riemsdijk WH, Nederlof M M, Kinniburgh D G and Koopal L K 1990 Analysis of ion binding on humic sub-stances and the determination of intrinsic affinity distributions. Analitica Chimica Acta 232, 189–207.
Devaux H 1916 Action rapide des solutions salines sur les plantes vivantes, deplacement reversible d'une partie des substances basiques contenues dans la plante. Compt. Rend. Acad. Sci. Paris 162, 561–563.
Duquette M and Hendershot W 1993 Soil surface charge evaluation by back-titration, I. Theory and method development. Soil Sci. Soc. Am. J. 57, 1222–1228.
Epstein E and Leggett J E 1954 The absorption of alkaline earth cations by barley roots. Kinetics and mechanism. Am. J. Bot. 41, 785–791.
Graham E R and Baker W L 1991 Ionic saturation of plant roots with special reference to hydrogen. Soil Sci. 72, 435–442.
Gray B, Drake M and Colby W G 1953 Potassium competition in grass-legume associations as a function of root cation exchange capacity. Soil Sci. Soc. Am. Proc. 17, 235–239.
Heintze S G 1964 Studies on cation exchange capacities of roots. Plant Soil 4, 365–383.
Hoagland D R and Broyer T C 1936 General nature of the process of salt accumulation by roots with description of experimental methods. Plant Physiol. 11, 471–507.
Huang C P 1981 The surface acidity of hydrous solids. In Ad-sorption of Inorganics at Solid-Liquid Interfaces. Eds. Anderson M A, Rubin A. pp. 183–218. Ann Arbor Sci. Co., Ann Arbor, Michigan.
Huffaker R C and Wallace A 1958 Possible relationships of cation-exchange capacity of plant roots to cation uptake. Soil Sci Soc. Am. Proc. 22, 392–394.
Ishikawa S, Wagatsuma T, Takano T, Tawaraya K, and Oomata K 2001a The plasma membrane intactness of root-tip cells is a primary factor for Al-tolerance in cultivars of five species. Soil Sci. Plant Nutr. 47, 489–501.
Ishikawa S, Wagatsuma T and Tawaraya K 2001b Effects of tempor-ary contact with Al ions on the plasma-membrane permeability of root-tip cells in seven plant species. In Plant nutrition – Food security and sustainability of agro-ecosystems. Eds. W J Horst et al. pp. 510–511. Kluwer Academic Press, The Netherlands.
Jenny H and Overstreet R 1939 Cation interchange between plant roots and soil colloids. Soil Sci. 54, 177–189.
Jozefaciuk G and Shin J S 1996 A modified back-titration method to measure soil titration curves minimizing soil acidity and dilution effects. Korean J. Soil Sci. and Fertilizer 29/4, 321–327.
Józefaciuk G and Szatanik-Kloc A 2001 Aluminium-induced changes in the surface and micropore properties of wheat roots: A study using the water vapor adsorption-desorption technique. Plant Soil 233, 95–108.
Jozefaciuk G and Szatanik-Kloc A 2002 Changes in specific area and energy of root surface of cereal plants in Al-solution cultures. Water vapor adsorption studies. Plant Soil 250, 129–140.
Keltjens W G 1995 Magnesium uptake by Al-stressed maize plants with special emphasis on cation interactions at root exchange sites. Plant Soil 171, 141–146.
Klein M and Horst W J 2001 Cation-specific exchange capacity of cell wall material isolated from roots of plant species differing in Al resistance. In Plant nutrition – Food security and sustainability of agro-ecosystems. Eds. W J Horst et al. pp. 266–267. Kluwer Academic Press, The Netherlands.
Knight A H, Crooke WM and Inkson R H E 1961 Cation-exchange capacities of tissues of higher and lower plants and heir related uronic acid contents. Nature 192, 142–143.
Koopal L K, Van Riemsdijk W K and Roffey M G 1987 Surface ionization and complexation models, a comparison of methods for determining model parameters. J. Colloid Interface Sci. 118, 117–136.
Marschner H, Romheld V 1983 In vivo measurement of root-induced pH changes at the soil-root interface: Effect of plant species and nitrogen source. Z. Pflanzenphysiol. 111, 249–254.
Mc Bride M 1997 A critique of diffuse double layer models applied to colloid and surface chemistry. Clays Clay Minerals 45, 598–608.
Morvan C, Demarty M and Thellier M 1979 Titration of isolated cell walls of Lemna minor L. Plant Physiol. 63, 1117–1122.
Mugwira L M and Elgawhary S M 1979 Aluminum accumulation and tolerance of trificale and wheat in relation to root cation exchange capacity. Soil Sci. Soc. Am. J. 43, 736–740.
Munn D A and McCollum R E 1976 Solution culture evaluation of sweet potato cultivar tolerance to aluminum. Agron. J. 68, 989–991.
Nederlof M M, De Wit J C, Riemsdijk W H and Koopal, L K 1993 Determination of proton affinity distributions for humic substances. Environ. Sci. Technol. 27, 846–856.
Ofei-Manu P, Wagatsuma T, Ishikawa S and Tawaraya K 2001 the plasma membrane strength of the root-tip cells and root phenolic compounds are correlated with Al tolerance in several common woody plants. Soil Sci. Plant Nutr. 47, 359–375.
Ramirez R, Rodriguez H H and Williams J E. 1986 Relacion entre la capacidad de intercambio cationico radical del sorgo y la tol-erancia a la toxicidad del aluminio. Agronomía Tropical. 36, 115–128.
Schmol N and Horst W J 2001 Cell-wall composition modulates aluminium toxicity. In Plant nutrition – Food security and sus-tainability of agro-ecosystems. Eds. WJ Horst et al. pp. 262–263. Kluwer Academic Publishers, The Netherlands.
Vedy J C and Bruckert S 1982 Soil solution; Composition and pe-dogenic significance. In Constituents and properties of soils. Eds. M Bonneau and B Souchier. pp. 184–214. Academic Press, N.Y.
Wagatsuma T 1983 Characterisation of adsorption sites for alu-minium in the roots. Soil Sci. Plant Nutr. 29, 499–415.
Wagatsuma T, Kaneko M and Hayasaka Y 1987 Destruction process of plant root cells by aluminium. Soil Sci. Plant Nutr. 33, 161–175.
Wagatsuma T and Akiba R 1989 Low surface negativity of root protoplasts from aluminum-tolerant plant species. Soil Sci. Plant Nutr. 35, 443–452.
Wiklander L and Elgabaly M M 1956 Relative uptake of adsorbed monovalent and divalent cations by excised barley roots as influenced by the exchange capacity. Soil Sci. 80, 91–93.
Williams D E and Coleman N T 1950 Cation exchange properties of plant root surfaces. Plant Soil 2, 243–256.
Woodward R A, Harper K T and Tiedemann A R 1984 An eco-logical consideration of the significance of cation-exchange capacity of roots of some Utah range plants. Plant Soil 78, 169–180.
Yermiyahu U, Brauer D K and Kinraide T B 1997b Sorption of aluminum to plasma membrane vesicles isolated from roots of Scout 66 and Atlas 66 cultivars of wheat. Plant Physiol. 115, 1119–1125.
Yermiyahu U, Rytwo G, Brauer D K and Kinraide T B 1997 Binding and electrostatic attraction of lanthanum (La3 +) and aluminum (Al3 +) to wheat root plasma membranes. J. Membrane Biol. 159, 239–252.
Zabowski D 1989 Limited release of soluble organics from roots during the centrifugal extraction of soil solutions. Soil Sci. Soc. Am. J. 53, 977–979.
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Jozefaciuk, G., Szatanik-Kloc, A. Decrease in variable charge and acidity of root surface under Al treatment are correlated with Al tolerance of cereal plants. Plant and Soil 260, 137–145 (2004). https://doi.org/10.1023/B:PLSO.0000030175.23904.5a
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DOI: https://doi.org/10.1023/B:PLSO.0000030175.23904.5a