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Diversification of Genes Encoding Mei2-Like RNA Binding Proteins in Plants

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Abstract

A predominantly plant-based family of genes encoding RNA binding proteins is defined by the presence of a highly conserved RNA binding motif first described in the mei2 gene of the fission yeast Schizosaccharomyces pombe. In silico analyses reveal nine mei2-like genes in Arabidopsis thaliana and six in Oryza sativa. These predicted genes group into four distinct clades, based on overall sequence similarity and subfamily-specific sequence elements. In situ analysis show that Arabidopsis genes from one of these clades, TEL1 and TEL2, are specifically expressed in central zone of the shoot apical meristem and the quiescent center of the root apical meristem, suggesting that they may somehow function to maintain indeterminacy in these tissues. By contrast, members of two sister clades, AML1 through AML5, are expressed more broadly, a trend that was confirmed by Q-PCR analysis. mei2-like transcripts with similar sequences showed similar expression patterns, suggesting functional redundancy within the four clades. Phenotypic analyses of lines that contain T-DNA insertions to individual mei2-like genes reveal no obvious phenotypes, further suggesting redundant activities for these gene products.

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  1. Daniel C. Jeffares

    Present address: Department of Evolutionary Biology, Universitetsparken 15, University of Copenhagen, DK-2100, Copenhagen Ø, Denmark

Authors and Affiliations

  1. Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY, 14853, USA

    Garrett H. Anderson & Maureen R. Hanson

  2. Institute of Molecular BioSciences, Massey University, Palmerston North, New Zealand

    Nena D.G. Alvarez, Carmel Gilman, Daniel C. Jeffares & Vernon C.W. Trainor

  3. Plant Breeding and Genomics, AgResearch, Private Bag 11008, Palmerston North, New Zealand

    Nena D.G. Alvarez

  4. Plant Breeding and Genomics, AgResearch, Private Bag 11008, Palmerston North, New Zealand

    Bruce Veit

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Anderson, G.H., Alvarez, N.D., Gilman, C. et al. Diversification of Genes Encoding Mei2-Like RNA Binding Proteins in Plants. Plant Mol Biol 54, 653–670 (2004). https://doi.org/10.1023/B:PLAN.0000040819.33383.b6

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