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Interaction of Photosystem 2-LHC2 Supercomplexes in Adjacent Layers of Stacked Chloroplast Thylakoid Membranes

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Photosynthetica

Abstract

Two Types of photosystem 2-light-harvesting complex 2 (PS2-LHC2) supercomplexes with similar pigment and protein composition were isolated directly from thylakoid membranes by sucrose density gradient centrifugation. Electron microscopy and single particle image analysis revealed the first Type as single unpaired PS2-LHC2 supercomplexes, whereas the second Type was characterized as pairs of two PS2-LHC2 supercomplexes attached together by their stromal sides. Unstacking of thylakoid membranes resulted in a spontaneous disintegration of the paired supercomplexes into single unpaired particles. A model of the organisation of the pigment-protein complexes in grana region is proposed.

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Authors and Affiliations

  1. Faculty of Biological Sciences, University of South Bohemia, Branišovská 31, 370 05 České Budějovice, Czech Republic

    L. Bumba

  2. Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, Branišovská 31, 370 05 České Budějovice, Czech Republic, e-mail

    L. Bumba & F. Vácha

  3. Institute of Physical Biology, University of South Bohemia, Branišovská 31, 370 05 České Budějovice, Czech Republic

    M. Hušák & F. Vácha

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  1. L. Bumba
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  2. M. Hušák
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  3. F. Vácha
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Bumba, L., Hušák, M. & Vácha, F. Interaction of Photosystem 2-LHC2 Supercomplexes in Adjacent Layers of Stacked Chloroplast Thylakoid Membranes. Photosynthetica 42, 193–199 (2004). https://doi.org/10.1023/B:PHOT.0000040590.10919.02

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