Abstract
Several inhibitors of deoxyhypusine synthase were examined for antifungalactivity. All compounds, used at concentrations of 1.0 mM or less, reduced mycelial growth of the oat leaf stripe pathogen Pyrenophora avenae grown on solid media. Four of the compounds completely inhibited fungal growth, 4a doing so at a concentration of only 0.61 mM. Inhibition of fungal growth afforded by someof the compounds was more marked than was reflected by the radial growth measurements.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Walters DR. The effects of a polyamine biosynthesis inhibitor on infection of Vicia faba L. by the rust fungus, Uromyces viciae-fabae (Pers.) Schroet. New Phytol 1986; 104: 613–619.
West HM, Walters DR. The effects of polyamine biosynthesis inhibitors on infection of Hordeum vulgare L. by Erysiphe graminis f. sp. hordei Marchal. New Phytol 1988; 110: 193–200.
Rajam MV, Galston AW. The effects of some polyamine biosynthetic inhibitors on growth and morphology of phytopathogenic fungi. Plant Cell Physiol 1985; 26: 683–692.
West HM, Walters DR. Effects of polyamine biosynthesis inhibitors on growth of Pyrenophora teres, Gaeumannomyces graminis, Fusarium culmorum and Septoria nodorum in vitro. Mycol Res 1989; 92: 453–457.
Porter CW, Sufrin JR. Interference with polyamine biosynthesis and/or function by analogs of polyamines or methionine332 as a potential anticancer chemotherapeutic strategy. Anticancer Res 1986; 6: 525–542.
Foster SA, Walters DR. Fungicidal activity of the putrescine analogue, keto-putrescine. Pesticide Sci 1993; 37: 267–272.
Havis ND, Walters DR, Foster SA, Martin WP, Cook FM, Robins DJ. Fungicidal activity of the synthetic putrescine analogue, (E)-1,4-diaminobut-2-ene, and derivatives. Pesticide Sci 1994a; 41: 61–69.
Havis ND, Walters DR, Martin WP, Cook FM, Robins DJ. Fungicidal activity of three putrescine analogues. Pesticide Sci 1994b; 41: 71–76.
Stevens L, Winther MD. Spermine, spermidine and putrescine in fungal development. Adv Microb Physiol 1979; 19: 63–148.
Mackintosh CA, Walters DR. Fungicidal activity of two spermidine analogues. J Phytopathology 1997a; 145: 43–45.
Mackintosh CA, Walters DR. Growth and polyamine metabolism in Pyrenophora avenae exposed to cyclohexylamine and norspermidine. Amino Acids 1997b; 13: 347–354.
Park MH, Wolff EC, Folk JE. Is hypusine essential for eukaryotic cell proliferation. TIBS 1993a; 18: 475–479.
Park MH, Wolff EC, Folk JE. Hypusine – Its posttranslational formation in eukaryotic initiation factor 5A and its potential role in cellular regulation. BioFactors 1993b; 4: 95–104.
Mandels GR. Kinetics of fungal growth. In: Ainsworth GC, Sussman AS, eds. The Fungi – Volume 1 – The Fungal Cell. London: Academic Press, 1965; 599–612.
Lee YB, Park MH, Folk JE. Diamine and triamine analogs and derivatives as inhibitors of deoxyhypusine synthase – Synthesis and biological activity. J Med Chem 1995; 38: 3053–3061.
Jakus J, Wolff EC, Park MH, Folk JE. Features of the spermidine-binding site of deoxyhypusine synthase as derived from inhibition studies. J Biol Chem 1993; 268: 13151–13159.
Park MH, Wolff EC, Lee YB, Folk JE. Antiproliferative effects of inhibitors of deoxyhypusine synthase. J Biol Chem 1994; 269: 27827–27832.
Wolff EC, Jakus J, Park MH, Folk JE. Inhibitors of deoxyhypusine synthase as potential regulators of hypusine biosynthesis in eIF-5A. FASEB J 1992; 6: A39.
Park MH, Wolff EC. Cell free synthesis of deoxyhypusine – Separation of protein substrate and enzyme and identification of 1,3-diaminopropane as a product of spermidine cleavage. J Biol Chem 1988; 263: 15264–15269.
Gerner EW, Mamont PS, Bernhardt A, Siat M. Posttranslational modification of the protein-synthesis initiation factor eIF-4D by spermidine in rat hepatoma cells. Biochemical J 1986; 239: 379–386.
Chen KY and Yang YC. Spermidine labels a 21,000-dalton protein in Neurospora crassa both in vivo and in vitro. FASEB J 1988; 2: A1563.
Yang YC, Chen KY, Seyfzadeh M, Davis RH. Deoxyhypusine hypusine formation on a 21,000-dalton cellular protein in a Neurospora crassa mutant in vivo and in vitro. Biochim Biophys Acta 1990; 1033: 133–138.
Sandholzer U, Cenea-Intemann M, Noegel AA, Lottspeich F. c-DNA and derived amino acid sequence of the hypusine containing protein from Dictyostelium discoideum. FEBS Lett 1989; 246: 93–100.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mackintosh, C.A., Walters, D.R. Antifungal Activity of Some Deoxyhypusine Synthase Inhibitors. Mycopathologia 156, 329–332 (2003). https://doi.org/10.1023/B:MYCO.0000003562.91682.53
Issue Date:
DOI: https://doi.org/10.1023/B:MYCO.0000003562.91682.53