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Conjugal Plasmid Transfer in Bacillus subtilis under Conditions of Soil Microcosms

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Abstract

Conjugal transfer of the small plasmid pUB110 betweenBacillus subtilis strains was studied under conditions of microcosms with sterile and nonsterile soil. Plasmid transfer proved to be possible after soil inoculation with vegetative partner cells or with their spores. Plasmid transfer occurred at temperatures of 30 and 22–23°C.

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REFERENCES

  1. Stotzky, G., Gene Transfer among Bacteria in Soil, Gene Transfer in the Environment, Levy, S.B. and Miller, R.V., Eds., New York: McGraw-Hill, 1989, pp. 165-222.

    Google Scholar 

  2. Hill, K. and Top, E., Gene Transfer in Soil Systems Using Microcosms, FEMS Microbiol. Ecol., 1998, vol. 25,no. 4, pp. 319-329.

    Google Scholar 

  3. Prozorov, A.A., Horizontal Gene Transfer in Bacteria: Laboratory Modeling, Natural Populations, and Data from Genome Analyses, Mikrobiologiya, 1999, vol. 68,no. 5, pp. 632-646.

    Google Scholar 

  4. Roberts, M. and Cohan, F., Recombination and Migration Rates in Natural Population of Bacillus subtilis and Bacillus mojaviensis, Evolution, 1995, vol. 49,no. 6, pp. 1081-1094.

    Google Scholar 

  5. Graham, J. and Istock, C., Genetic Exchange in Bacillus subtilis in Soil, Mol. Gen. Genet., 1978, vol. 166,no. 3, pp. 287-290.

    Google Scholar 

  6. Graham, J. and Istock, C., Parasexuality and Microevolution in Experimental Populations of Bacillus subtilis, Evolution, 1981, vol. 35,no. 5, pp. 954-963.

    Google Scholar 

  7. Jarrett, P. and Stephenson, M., Plasmid Transfer between Strains of Bacillus thuringiensis Infecting Gallea melonella and Spodoptera littoralis, Appl. Environ. Microbiol., 1990, vol. 56,no. 5, pp. 1608-1614.

    Google Scholar 

  8. Thomas, D.J., Morgan, J., Whipps, J., and Saunders, J., Plasmid Transfer between the Bacillus thuringiensis Subspecies kurstaki and tenebrionis in Laboratory Culture and Soil and in Lepidopteran and Coleopteran Larvae, Appl. Environ. Microbiol., 2000, vol. 66,no. 1, pp. 118-124.

    Google Scholar 

  9. Thomas, D.J., Morgan, J., Wipps, J., and Saunders, J., Plasmid Transfer between the Bacillus thuringiensis subsp. israelensis Strains in Laboratory Culture, River Water, and Dipteran Larvae, Appl. Environ. Microbiol., 2001, vol. 67,no. 1, pp. 330-338.

    Google Scholar 

  10. Lotareva, O.V., Nezametdinova, V.Z., Fedorina, E.V., Poluektova, E.U., Titok, M.A., and Prozorov, A.A., Conjugal Mobilization Realized with a High Frequency by a Natural Stain of Bacillus subtilis Harboring a Large Plasmid, Genetika, 2001, vol. 37,no. 12, pp. 1598-1603.

    Google Scholar 

  11. Lotareva, O.V., Nezametdinova, V.Z., Fedorina, E.A., and Prozorov, A.A., Behavior of Various Plasmids in the Soil Strain Bacillus subtilis 19 Capable of Conjugation, Mikrobiologiya, 2002, vol. 71,no. 4, pp. 567-569.

    Google Scholar 

  12. Gagarina, E.Yu., A Study of Small Cryptic Plasmids Discovered in Soil Strains of Bacillus subtilis, Cand. Sci. (Biol.) Dissertation, Moscow: Vavilov Inst. Gen. Genet., Russ. Acad. Sci., 2002.

    Google Scholar 

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Authors and Affiliations

  1. Vavilov Institute of General Genetics, Russian Academy of Sciences, ul. Gubkina 3, Moscow, 119899, Russia

    O. V. Lotareva & A. A. Prozorov

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  1. O. V. Lotareva
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  2. A. A. Prozorov
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Lotareva, O.V., Prozorov, A.A. Conjugal Plasmid Transfer in Bacillus subtilis under Conditions of Soil Microcosms. Microbiology 72, 690–693 (2003). https://doi.org/10.1023/B:MICI.0000008371.18221.45

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  • DOI: https://doi.org/10.1023/B:MICI.0000008371.18221.45

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