Abstract
We investigated the dissociation of single-ring heptameric GroEL (SR1) by high hydrostatic pressure in the range 0.5–3.0 kbar. The kinetics were studied as a function of temperature in the range 15–35 °C. The dissociation processes at each pressure and temperature showed biphasic behavior. The slower rate (k 1,obs) was confirmed to be the self-dissociation of SR1 at any specific temperature at atmospheric pressure. This dissociation was pressure independent and followed concentration-dependent first-order kinetics. The self-dissociation rates followed normal Eyring plots (ln k 1,obs/T vs. 1/T) from which the free energy of activation (ΔG ≠ = 22 ± 0.3 kcal mol−1), enthalpy of activation (ΔH ≠ = 18 ± 0.5 kcal mol−1), and entropy of activation (ΔS ≠ = −15 ± 1 kcal mol−1) were evaluated. The effect of pressure on the dissociation rates resulted in nonlinear behavior (ln k 2,obs vs. pressure) at all the temperatures studied indicating that the activation volumes were pressure dependent. Activation volumes at zero pressure (V ≠ o) and compressibility factors (β≠) for the dissociation rates at the specific temperatures were calculated. This is the first systematic study where the self-dissociation of an oligomeric chaperonin as well as its activation parameters are reported.
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Panda, M., Horowitz, P.M. Activation Parameters for the Spontaneous and Pressure-Induced Phases of the Dissociation of Single-Ring GroEL (SR1) Chaperonin. J Protein Chem 23, 85–94 (2004). https://doi.org/10.1023/B:JOPC.0000016262.27420.3a
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DOI: https://doi.org/10.1023/B:JOPC.0000016262.27420.3a