Abstract
Brief exposures of male Choristoneura rosaceana and Argyrotaenia velutinana to the plumes generated by lures releasing 3-component pheromone blends specifically tuned for each species or by commercially distributed Isomate OBLR/PLR Plus pheromone “rope” dispensers induced markedly different subsequent behavioral responses to pheromone. A greater proportion of C. rosaceana males took flight and successfully oriented toward lures 24 h after preexposure to a lure, a rope, or the lure–rope combination in a sustained-flight wind tunnel compared to naïve moths. Flights were also longer for preexposed than naïve moths. Preexposed male C. rosaceana were not more likely to fly toward ropes 24 h after preexposure. By contrast, fewer male A. velutinana oriented to lures 24 h after preexposure than did naïve moths. Those preexposed A. velutinana successfully locking onto plumes from lures flew for significantly shorter intervals than did unexposed moths. Electroantennograms revealed no changes at the periphery 15 min and 24 h after preexposure. For A. velutinana, the long-lasting effect was decreased attraction to a lure and increased attraction to a rope. For C. rosaceana, pheromone preexposure increased responsiveness to its authentic blend. This behavioral evidence is sufficient to explain why sexual communication of C. rosaceana is more difficult to disrupt than that of A. velutinana. Furthermore, it suggests a more complete blend of pheromone may be necessary to disrupt the former species but not the latter when using rope dispensers.
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REFERENCES
Agnello, A. M., Reissig, W. H., Spangler, S. M., Charlton, R. E., and Kain, D. P. (1996). Trap response and fruit damage by obliquebanded leafroller (Lepidoptera: Torticidae) in pheromone-treated apple orchards in New York. Environ. Entomol. 25: 268–282.
Anderson, P., Sadek, M. M., and Hansson, B. S. (2003). Pre-exposure modulates attraction to sex pheromone in a moth. Chem. Senses. 28: 285–291.
Baker, T. C., and Cardé, R. T. (1979). Analysis of pheromone-mediated behavior in male Grapholita molesta, the Oriental fruit moth (Lepidoptera: Tortricidae). Environ. Entomol. 8: 956–968.
Bartell, R. J. (1982). Mechanisms of communication disruption by pheromone in control of Lepidoptera: A review. Physiol. Entomol. 7: 353–364.
Bartell, R. J., and Lawrence, L.A. (1976a). Reduction in responsiveness of male light-brown apple moth to sex pheromone following previous brief pheromonal exposure is concentration dependent. J. Aust. Entomol. Soc. 15: 236.
Bartell, R. J., and Lawrence, L.A. (1976b). Reduction in responsiveness of Epiphyyas postvittana (Lepidoptera) to sex pheromone following pulsed pheromonal exposure. Physiol. Entomol. 2: 1–6.
Bartell, R. J., and Lawrence, L.A. (1976c). Reduction in responsiveness of Epiphyyas postvittana (Lepidoptera) to sex pheromone following pulsed pre-exposure to pheromone components. Physiol. Entomol. 2: 89–95.
Bartell, R. J., and Roelofs, W. L. (1973). Inhibition of sexual response in males of the moth Argyrotaenia velutinana by brief exposures to synthetic pheromone and its geometric isomer. J. Insect Physiol. 19: 655–661.
Bell, J. S., and Heisenberg, M. (1994). Associative odor learning in Drosophila abolished by chemical ablation of mushroom bodies. Science. 263: 692–695.
Cardé, R. T. (1990). Principles of mating disruption. In Ridgway, R. L., and Silverstein, R. M. (eds.), Behavior-Modifying Chemicals for Pest Management: Applications of Pheromones and other Attractants, Marcel Dekker, New York. pp. 47–71.
Cardé, R. T., Comeau, A., Baker, T. C., and Roelofs, W. L. (1975). Moth mating periodicity: Temperature regulates the circadian gate. Experientia. 31: 46–48.
Cardé, R. T., Mafra-Neto, A., Staten, R. T., and Kuenen, L. P. S. (1997). Understanding mating disruption in the pink bollworm moth. IOBC/WPRS Bull. 20(1): 191–201.
Cardé, R. T., and Minks, A. K. (1995). Control of moth pests by mating disruption: Successes and constraints. Ann. Rev. Entomol. 40: 559–585.
Cardé, R. T., Staten, R. T., and Mafra-Neto, A. (1998). Behavior of pink bollworm males near high-dose, point sources of pheromone in field wind tunnels: Insights into mechanisms of mating disruption. Entomol. Exp. Appl. 89: 35–46.
Deland, J.-P., Judd, G. J. R., and Roitberg, B.D. (1994). Disruption of pheromone communication in thee sympatric leafroller (Lepidoptera: Tortricidae) pests of apple in British Columbia. Environ. Entomol. 23: 1084–1090.
Evenden, M. L., Judd, G. J. R., and Borden, J. H. (1999a). Pheromone-mediated mating disruption of Choristoneura rosaceana: Is the most attractive blend really the most effective? Entomol. Exp. Appl. 90: 37–47.
Evenden, M. L., Judd, G. J. R., and Borden, J. H. (1999b). Mating disruption of two sympatric, orchard inhabiting tortricids, Choristoneura rosaceana and Pandemis limitata (Lepidoptera: Tortricidae), with pheromone components of both species' blends. J. Econ. Entomol. 92: 380–390.
Evenden, M. L., Judd, G. J. R., and Borden, J. H. (1999c). Simultaneous disruption of pheromone communication in Choristoneura rosaceana and Pandemis limitata with pheromone and antagonist blends. J. Chem. Ecol. 25: 501–517.
Evenden, M. L., Judd, G. J. R., and Borden, J. H. (2000). Investigations of mechanisms of pheromone communication disruption of Choristoneura rosaceana (Harris) in a wind tunnel. J. Insect Behav. 13: 499–510.
Figueredo, A. J., and Baker, T. C. (1992). Reduction of the response to sex pheromone in the oriental fruit moth, Grapholita molesta (Lepidoptera: Tortricidae) following successive pheromonal exposures. J. Insect Behav. 5: 347–362.
Gut, L. J., Stelinski, L. L., Thompson, D. R., and Miller, J. R. (2004). Behavior modifying chemicals: Prospects and constraints in IPM. In: Koul, O., Dhaliwal, G. S., and Cuperus, G. W. (eds.), Integrated Pest Management-Potential, Constraints, and Challenges, CABI Press, Wallingford, UK, pp. 73–121.
Hill, A. S., and Roelofs, W. L. (1979). Sex pheromone components of the obliquebanded leafroller moth, Choristoneura rosaceana. J. Chem. Ecol. 5: 3–11.
Knight, A. L., Thomson, D. R., and Cockfield, S. D. (1998). Developing mating disruption of obliquebanded leafroller (Lepidoptera: Tortricidae) in Washington State. Environ. Entomol. 27: 1080–1088.
Knight, A. L., and Turner, J. E. (1999). Mating disruption of Pandemis spp. Lepidoptera: Torticidae). Environ. Entomol. 28: 81–87.
Kuenen, L. P. S., and Baker, T. C. (1981). Habituation versus sensory adaptation as the cause of reduced attraction following pulsed and constant sex pheromone pre-exposure in Trichoplusia ni. J. Insect Physiol. 27: 721–726.
Lawson, D. S., Reissig, W.H, Agnello, A. M., Nyrop, J. P., and Roelofs, W. L. (1996). Interference with the mate-finding communication system of the obliquebanded leafroller (Lepidoptera: Tortricidae) using synthetic sex pheromones. Environ. Entomol. 25: 895–905.
Linn, C. E. (1997). Neuroendocrine factors in the photoperiodic control of male moth responsiveness to sex pheromone. In Cardé, R. T., and Minks, A. K. (eds.), Insect Pheromone Research, New Directions, Chapman and Hall, New York, pp. 194–209.
Linn, C. E., Campbell, M. G., and Roelofs, W. L. (1992). Photoperiod cues and the modulatory action of octopamine and 5-hydroxytryptamine on locomotor and pheromone response in male gypsy moth, Lymantria dispar. Arch. Insect Biochem. Physiol. 20: 265–283.
Linn, C. E., and Roelofs, W. L. (1981). Modification of sex pheromone blend discrimination in male Oriental fruit moth by pre-exposure to (E)-8-dodecenyl acetate. Physiol. Entomol. 6: 421–429.
Miller, J. R., and Roelofs, W. L. (1978). Sustained-flight tunnel for measuring insect responses to wind-borne sex pheromones. J. Chem. Ecol. 4: 187–198.
Nagata, K. (1989). Revue: Pest control by mating disruption in Japan. Jpn. Pestic. Inf. 54: 3–6.
Novak, M. A., Reissig, W. H., and Roelofs, W. L. (1978). Orientation disruption of Argyrotaenia velutinana and Choristoneura rosaceana (Lepidoptera: Tortricidae) male-moths. J. N. Y. Entomol. Soc. 4: 311–315.
Novak, M. A., and Roelofs, W. L. (1985). Behavior of male redbanded leafroller moth, Argyrotaenia velutinana (Lepidoptera: Tortricidae), in small disruption plots. Environ. Entomol. 14: 12–16.
Pophof, B. (2000). Octopamine modulates the sensitivity of silkmoth pheromone receptor neurons. J. Comp. Phys. A. 186: 307–313.
Pophof, B. (2002). Octopamine enhances moth olfactory responses to pheromones, but not those to general odorants. J. Comp. Phys. A. 188: 659–662.
Reissig, W. H., Novak, M., and Roelofs, W. L. (1978). Orientation disruption of Argyrotaenia velutinana and Choristoneura rosaceana male moths. Environ. Entomol. 7: 631–635.
Roelofs, W. H., Hill, A., and Cardé, R. T. (1975). Sex pheromone components of the redbanded leafroller, Argyrotaenia velutinana (Lepidoptera: Tortricidae). J. Chem. Ecol. 1: 83–89.
Sanders, C. J. (1984). Sex pheromone of the spruce budworm (Lepidoptera: Tortricidae): Evidence for a missing component. Can. Entomol. 116: 93–100.
Sanders, C. J. (1985). Disruption of spruce budworm, Choristoneura fumiferana (Lepidotera: Tortricidae), mating in a wind tunnel by synthetic pheromone: Role of habituation. Can. Entomol. 117: 391–393.
Sanders, C. J. (1995). Disruption of male spruce budworm orientation to female moths by pheromone and pheromone analogues in a wind tunnel. Physiol. Entomol. 89: 71–80.
Sanders, C. J. (1996). Effects of prolonged exposure to different concentrations of synthetic pheromone on mating disruption of spruce budworm moths in a wind tunnel. Can. Entomol. 128: 57–66.
Sanders, C. J. (1998). Effect of pheromone permeation on sustained flight of male spruce budworm. Can. Entomol. 130: 539–544.
SAS Institute (1989). SAS/STAT User's Guide, version 6, 4th ed., Vol. 1, SAS Institute, Cary, NC.
Seabrook, W. D., and Kipp, L. R. (1986). The use of a two component blend of the spruce budworm sex pheromone for mating suppression. In Chaudry, I. A., and Thies, C. (eds.), Proceedings, 13th International Symposium on Controlled Release of Biorational Materials. Norfolk, Virginia, pp. 128–129.
Shorey, H. H., and Hale, R. L. (1965). Mass rearing of the larvae of nine noctuid species on a simple artificial medium. J. Econ. Entomol. 58: 522–524.
Sokal, R. R., and Rohlf, F. J. (1981). Biometry, W. H. Freeman, New York.
Stelinski, L. L., Gut, L. J., and Miller, J. R. (2003b). Concentration of air-borne pheromone required for long-lasting peripheral adaptation in the obliquebanded leafroller, Choristoneura rosaceana. Physiol. Entomol. 28: 97–107.
Stelinski, L. L., Miller, J. R., and Gut, L. J. (2003a). Presence of long-lasting peripheral adaptation in the obliquebanded leafroller, Choristoneura rosaceana and absence of such adaptation in the redbanded leafroller, Argyrotaenia velutinana. J. Chem. Ecol. 29: 403–422.
Stelinski, L. L., Miller, J. R., Ressa, N. E., and Gut, L. J. (2003c). Increased EAG responses of tortricid moths after prolonged exposure to plant volatiles: Evidence for octopaminemediated sensitization. J. Insect Plysiol. 49: 845–856.
Thomson, D. R., Angerilli, N. P.D., Vincent, C., and Gaunce, A. P. (1991). Evidence for regional differences in the response of obliquebanded leafroller (Lepidoptera: Tortricidae) to sex pheromone blends. Environ. Entomol. 20: 935–938.
Vakenti, J. M., Gaunce. A.P., Slessor, K.N., King, G.G. S., Allan, S. A., Madsen, H.F., and Borden, J. H. (1988). Sex pheromone components of the oblique-banded leafroller, Choristoneura rosaceana in the Okanagan Valley of British Colombia. J. Chem. Ecol. 14: 605–621.
Yin, J. C. P., Wallach, J. S., del Vecchio, M., Wilder, E. L., Zhou, H., and Tully. T. (1994). Induction of a dominant negative CREB transgene specifically blocks long-term memory in Drosophila. Cell. 79: 49–58.
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Stelinski, L.L., Gut, L.J., Vogel, K.J. et al. Behaviors of Naïve vs. Pheromone-Exposed Leafroller Moths in Plumes from High-Dosage Pheromone Dispensers in a Sustained-Flight Wind Tunnel: Implications for Mating Disruption of These Species. Journal of Insect Behavior 17, 533–554 (2004). https://doi.org/10.1023/B:JOIR.0000042540.09188.eb
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DOI: https://doi.org/10.1023/B:JOIR.0000042540.09188.eb