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Comparative Study of Substrate–Inhibitor Specificity of Brain Cholinesterase Activity of Pacific Salmon Fish of the Salmonidae Family

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Abstract

Salmon fish of the Salmonidae family, representatives of the Pacific salmon Oncorhynchus genus, the humpback salmon O. gorbuscha Wielb., dog salmon O. keta, coho salmon O. kisutch, blueback salmon O. nerka, and chinook salmon O. tschawytscha, and also of noble salmons genus, Salmo—black salmon S. salar, caught in the northwest water area of the Pacific ocean, are studied in the work. Iodides of acetyl-, propionyl-, and butyrylthiocholine were used as substrates. Carbamate proserine and 5 organophosphorus inhibitors were studied as inhibitors. Testing of homogeneity of cholinesterase activity in the brain tissue has revealed only one enzyme in the coho salmon, while several enzymes, in the dog salmon and humpback salmon, which can be an enzymologic argument for the favor of hypothesis about the presence of interspecies groups among Pacific salmons of the Oncorhynchus genus. Interspecies differences in substrate specificity of the brain tissue in the studied salmonid species are found. The fish enzymes of the fishes have shown a high sensitivity to proserine. Only in the case of diisopropylfluorophosphate, both interspecies and intergenus differences are revealed.

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Authors and Affiliations

  1. Pacific Research Fishery Center, Vladivostok, Russia

    N. N. Kovalev & E.V. Rozengart

  2. Pacific Research Fishery Center, Vladivostok, Russia

    A. I. Chepkasova

Authors
  1. N. N. Kovalev
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  2. E.V. Rozengart
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  3. A. I. Chepkasova
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Kovalev, N.N., Rozengart, E. & Chepkasova, A.I. Comparative Study of Substrate–Inhibitor Specificity of Brain Cholinesterase Activity of Pacific Salmon Fish of the Salmonidae Family. Journal of Evolutionary Biochemistry and Physiology 40, 258–263 (2004). https://doi.org/10.1023/B:JOEY.0000042628.79644.1e

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  • DOI: https://doi.org/10.1023/B:JOEY.0000042628.79644.1e

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