Abstract
Behavioral bioassays have confirmed that the oviposition-deterring secretion of the cabbage seed weevil, Ceutorhynchus assimilis Payk., can be isolated from glass tubes marked by the weevil and from extracts of its dissected seventh urotergite. Analysis of the secretion by gas chromatography—mass spectrometry showed that it contained iso- and n-alkanes, dimethylalkanes, alkenes, fatty acids, 15-nonacosanonc, 15-nonacosanol, and cholesterol. The oviposition-deterring properties of the secretion are associated with a more polar traction, isolated by liquid chromatography front an extract of the seventh urotergite.
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REFERENCES
BEHAN, M., and SCHOONHOVEN, L. M. 1978. Chemoreception of an oviposition deterrent associated with eggs in Pieris brassicae. Entomol. Exp. Appl. 24:163–179
DOOLITTLE, R. E., PROVEAUX, A. T., ALBORN, H. T., and HEATH, R. R. 1995. Quadruple storage mass spectrometry of mono-and dimethylalkanes. J. Chem. Ecol. 21:1677–1695
EVERSHED, R. P., HERON, C., CHARTERS, S., and GOAD, L. J. 1992. The survival of food residues: New methods of analysis, interpretation and application. Proc. Br. Acad. 77:187–208
FERGUSON, A. W., and WILLIAMS, I. H. 1991. Deposition and longevity of oviposition-deterring pheromone in the cabbage seed weevil. Physiol. Entomol. 1:27–33
KOZLOWSKI, M. W. 1991. Oviposition marker in the cabbage seed weevil, Ceutorhynchus assimilis, remarks on a film: “Dance for the new generation.” IOBC/WPRS Bull. 14(6):219–222
KOZLOWSKI, M. W., LUX, S., and DMOCH, J. 1983. Oviposition behaviour and pod marking in the cabbage seed weevil, Ceutorhynchus assimilis. Entomol. Exp. Appl. 34:277–282
JEANNEL, R. 1949. Ordre des Coleoptères, pp. 771–1077 in P. P. Grasse (ed.). Traite de Zoologie, Vol. 9, Masson, Paris
LIAS, S. G., and STEIN, S. S. 1990. NIST/EPA/MSDC Mass Spectral Database. NIST, Gaithersburg, Maryland
MCCARTHY, E. D., HAN, J., and CALVIN, M. 1968. Hydrogen atom transfer in mass spectrometric fragmentation patterns of saturated aliphatic hydrocarbons. Anal. Chem. 40:1475–1480
POMONIS, J. G., FATLAND, C. F., NELSON, D. R., and ZAYLASKIE R. G. 1978. Insect hydrocarbons 1. Corroboration of structure by synthesis and mass spectrometry of mono-and dimethylalkanes. J. Chem. Ecol. 4:27–39
POMONIS, J. G., NELSON, D. R., and FATLAND, C. L. 1980. Insect hydrocarbons 2. Mass spectra of dimethylalkanes and the effect of the number of methylene groups between methyl groups on fragmentation. J. Chem. Ecol. 6:965–972
PROKOPY, R. J., AVERILL, A. L., BARDINELLI, C. M., BOWDAN, E. S., COOLEY, S. S., CRNJAR, R. M., DUNDULIS, E. A., ROITBERG, C. A., SPATCHER, P. J., TUMLINSON, J. H., and WEEKS, B. L. 1982. Site of production of an oviposition-deterring pheromone component in Rhagoletis pomonella flies. J. Insect Physiol. 28:1–10
PROKOPY, R. J., ROITBERG, B. D., and AVERILL, A. L. 1984. Resource partitioning, pp. 301–330, in W. J. Bell and R. T. Carde (eds.). Chemical Ecology of Insects. Chapman and Hall, London
RICHTER, I., and KRAIN, H. 1981. Cuticular lipid constituents of cabbage seedpod weevils and host plant oviposition sites as potential pheromones. Lipids 15:580–586
ROITBERG, B. D., and MANGEL, M. 1988. On the evolutionary ecology of host-marking pheromones. Evol. Ecol. 2:289–315
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Mudd, A., Ferguson, A.W., Blight, M.M. et al. Extraction, Isolation, and Composition of Oviposition-Deterring Secretion of Cabbage Seed Weevil Ceutorhynchus assimilis . J Chem Ecol 23, 2227–2240 (1997). https://doi.org/10.1023/B:JOEC.0000006441.74950.2f
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DOI: https://doi.org/10.1023/B:JOEC.0000006441.74950.2f