Abstract
Glasses incorporating increasing amounts of bovine serum albumin were prepared by sol–gel techniques from a tetra methoxy silane precursor. The surface of the glass samples was studied by X-ray photoelectron spectroscopy, revealing that the protein is present also in the superficial layer of the silica network. Moreover, the protein is distributed in a dose-dependent way, since the N/Si atomic ratio increases linearly with the albumin concentration in the reaction mixture. Angle-dependent measurements show that the protein distribution occurs homogeneously and is the same at different sampling depths. Protein incorporation in the bulk SiO2 network, with a uniform protein distribution between bulk and surface, is confirmed by infrared spectroscopy measurements, performed both in reflectance and transmittance mode. The reaction with a specific antibody and the adhesivity assay of osteoblastic cells show that embedded albumin present on the glass surface is able to interact with other proteins.
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Iucci, G., Infante, G., Rossi, L. et al. Albumin-containing sol–gel glasses: Chemical and biological study. Journal of Materials Science: Materials in Medicine 15, 601–606 (2004). https://doi.org/10.1023/B:JMSM.0000026381.58969.e9
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DOI: https://doi.org/10.1023/B:JMSM.0000026381.58969.e9