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The Effect of a Two-Hour, Room Temperature Incubation of Human Spermatozoa in TEST-Yolk Buffer on the Rate of Fertilization in Vitro

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Abstract

Patients: To reassess the use of TEST-yolk buffer (TYB) in an in vitro fertilization (IVF) program by comparing fertilization rates achieved in a glucose-free cleavage medium by the standard IVF preparation of sperm versus a 2-h, room temperature incubation of sperm in TYB.

Methods: Oocytes collected for IVF were randomly split into two groups and inseminated with either TYB-treated sperm or IVF-prepared sperm.

Setting: Stanford Reproductive Endocrinology and Infertility Center.

Patients: Fifty couples undergoing IVF with at least 10 mature oocytes. Main Outcome Measures: Fertilization rates in vitro.

Results: Fertilization rates were significantly higher (p=0.015) with TYB treatment. The average 2PN fertilization rate was 49.6% (188/379) for the IVF group and 57.4% (221/385) in the IVF with TYB group.

Conclusions: A 2-h, room temperature incubation of sperm in TYB produces significantly higher 2PN fertilization rates as compared to standard IVF preparation of sperm in a current generation cleavage medium.

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Authors and Affiliations

  1. California Polytechnic State University, San Luis Obispo, California

    Julie R. Jensen & John H. Walker

  2. Department of Obstetrics and Gynecology, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, California, 94305-5317

    Amin A. Milki, Lynn Westphal & Barry Behr

Authors
  1. Julie R. Jensen
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  2. John H. Walker
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  3. Amin A. Milki
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  4. Lynn Westphal
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  5. Barry Behr
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Correspondence to Barry Behr.

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Jensen, J.R., Walker, J.H., Milki, A.A. et al. The Effect of a Two-Hour, Room Temperature Incubation of Human Spermatozoa in TEST-Yolk Buffer on the Rate of Fertilization in Vitro. J Assist Reprod Genet 21, 169–173 (2004). https://doi.org/10.1023/B:JARG.0000031250.35595.ed

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  • DOI: https://doi.org/10.1023/B:JARG.0000031250.35595.ed

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