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Reversed-Phase High-Performance Liquid Chromatography of Hemin-Based Peptides on a Sorbent Modified with Zinc 4,5-Carboxyphthalocyaninate

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Abstract

The chromatographic retention of some hemin-based peptides on the Diasphere-110-C18 sorbent modified with zinc octa-4,5-carboxyphthalocyaninate was studied. It was demonstrated that this stationary phase is more selective for hemin-peptides than Nucleosil-C18 and Silasorb-600. The conditions of the separation of mixtures of hemin-based peptides were determined, and the parameters of their chromatographic separation were calculated. It was shown that the retention parameters are related to the structure of hemin-based peptides.

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Authors and Affiliations

  1. Department of Chemistry, Moscow State University, Vorob'evy gory, Moscow, 119899, Russia

    G. D. Brykina, S. M. Matusova & O. A. Shpigun

  2. Lomonosov State Academy of Fine Chemical Technology, pr. Vernadskogo 86, Moscow, 117571, Russia

    T. V. Zarubina, G. A. Zheltukhina & R. P. Evstigneeva

Authors
  1. G. D. Brykina
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  2. S. M. Matusova
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  3. O. A. Shpigun
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  4. T. V. Zarubina
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  5. G. A. Zheltukhina
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  6. R. P. Evstigneeva
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Brykina, G.D., Matusova, S.M., Shpigun, O.A. et al. Reversed-Phase High-Performance Liquid Chromatography of Hemin-Based Peptides on a Sorbent Modified with Zinc 4,5-Carboxyphthalocyaninate. Journal of Analytical Chemistry 59, 266–269 (2004). https://doi.org/10.1023/B:JANC.0000018971.44766.46

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  • DOI: https://doi.org/10.1023/B:JANC.0000018971.44766.46

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