Abstract
The effects of enhanced UV-B radiation on hormone changes in vegetative and reproductive tissues of tomato (Lycopersicon esculentum Mill.) and their relationships with reproductive characteristics were studied. Two cultivars, TongHui (TH) and XiaGuang (XG), were grown in the field for one growing season under ambient (Control), ambient plus 2.54 kJ m−2 d−1 (T1) or ambient plus 4.25 kJ m−2 d−1 (T2) of supplemental ultraviolet-B (280–320 nm). The number of open flowers increased significantly in the TH cultivar under T2 while it declined in the XG cultivar under T1. Although pollen germination from both cultivars was inhibited by UV-B treatment, fruit number was enhanced in the TH cultivar at both UV-B doses and in the XG cultivar at the low dose. On the other hand, seed size (dry weight) was reduced in the XG cultivar by both UV-B doses and in the TH cultivar at the low UV-B dose. The final germination rates of seeds from control and UV-B treated plants of both cultivars showed no significant differences (p > 0.05), while germination was delayed in the TH cultivar at both doses of UV-B and in the XG cultivar only for T2. To determine the mechanism of UV-B's effects on developmental processes, hormone concentrations in leaves, pistils and seeds were analyzed using ELISA on partially purified extracts. The results suggested that enhanced UV-B radiation induced hormone changes in both vegetative and reproductive tissues. The alteration of flower number may be associated with the changes of ZR in leaves under enhanced UV-B radiation and the delayed germination may due to the changes in seed ABA and GAs.
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References
Allen D., Nogués S.and Baker N.R.1998.Ozone depletion and increased UV-B radiation:is there a real threat to photosynthesis.J.Exp.Bot.49:1775–1788.
Applewhite P.B., Sawhney R.and Galston A.W.1994.Isatin as an auxin source favoring.oral and vegetative shoot regeneration from calli produced by thin layer explants of tomato pedicel.Plant Growth Regul.15:17–21.
Balbi V.and Lomax T.L.2003.Regulation of early tomato fruit development by the diageotropica gene.Plant Physiol.131: 186–197.
Barsig M.and Malz R.2000.Fine structure,carbohydrates and photosynthetic pigments of sugar maize leaves under UV-B radiation.Environ.Exp.Bot.43:121–130.
Bjorn L.O.and Murphy T.M.1985.Computer calculation of solar ultraviolet radiation at ground level.Physiol.Veg.23: 555–561.
Caldwell M.M.1971.Solar UV-B irradiation and the growth and development of higher plants.In:Giese A.C.(eds), Photophysiology,Vol.6.Academic Press, New York,NY, pp.131–171.
Caldwell M.M., Bjorn L.O., Bornman J.F., Flint S.D.and Kulandaivelu G.1998.Effects of increased solar ultraviolet radiation on terrestrial ecosystems.J.Photochem.Photobiol. B:Biol.46:40–52.
Davies P.J.1995.The plant hormones:their nature,occurrence and functions.In:Davies P.J.(ed.),Plant Hormones:Physiology,Biochemistry and Molecular Biology.Kluwer Academic Publishers, The Netherlands,pp.1–12.
Day T.A.and Demchik S.M.1996.In.uence of enhanced UV-B radiation on biomass allocation and pigment concentrations in leaves and reproductive structures of greenhouse-grown Brassica rapa.Vegetation 127:109–116.
Demchik S.M.and Day T.A.1996.Effect of enhanced UV-B radiation on pollen quantity,quality and seed yield in Brassica rapa.Am.J.Bot.83:573–579.
Feng H., An L., Tan L., Hou Z.and Wang X.2000.Effect of enhanced ultraviolet-B radiation on pollen germination and tube growth of 19 taxa in vitro.Environ.Exp.Bot.42:45–53.
Flint S.D.and Caldwell M.M.1984.Partial inhibition of intro pollen germination by simulated solar ultraviolet-B radiation. Ecology 65:792–795.
Grammatikopoulos G., Karousou R., Kokkini S.and Manetas Y.1998.differential Effects of enhanced UV-B radiation on reproductive e.ort in two chemotypes of Mentha spicata under eld conditions.Aust.J.Plant Physiol.25:345–351.
Green A.E.S., Cross K.R.and Smith L.A.1980.Improved analytic characterization of ultraviolet skylight.Photochem. Photobiol.31:59–65.
Gustafson F.G.1937.Parthenocarpy induced by pollen extracts.Am.J.Bot.24:102–107.
Huang S., Dai Q., Peng S., Chavez A.Q., Miranda M.L.L., Visperas R.M.and Vergara B.S.1997.In.uence of supplemental ultraviolet-B on indoleacetic acid and calmodulin in the leaves of rice (Oryza sativa L.).Plant Growth Regul.21:59–64.
Jansen M.A., den Noort R.E., Tan M.Y., Prinsen E., Lagrimini L.M.and Thorneley R.N.2001.Phenoloxidizing peroxidases contribute to the protection of plants from ultraviolet radiation stress.Plant Physiol.126:1012–1023.
Li G.J., Wang S.C., Xia K.and Zhou X.2003.Effect of yeast elicitor and salicylic acid on the.uctuation of phytohormone contents in Ti-transformed Salvia miltiorrhiza cell cultures. Plant Growth Regul.39:27–32.
Li Y., Yue M.and Wang X.1998.Effects of enhanced ultraviolet-B radiation on crop structure,growth and yield components of spring wheat under eld conditions.Field Crops Res.57:253–263.
Macherness S.A.H.2000.Plant responses to ultraviolet-b (UV-B:280-320 nm)stress:what are the key regulators?Plant Growth Regul.32:27–39.
Mark U., Saile-Mark M.and Tevini M.1996.Effects of solar UV-B radiation on growth,flowering,and yield of central and southern European maize cultivars (Zea mays L.).Photochem.Photobiol.64:457–463.
Mazza C.A., Battista D., Zima A.M., Szwarcberg-Bracchitta M., Giordano C.V., Acevedo A., Scopel A.L.and Ballare L.1999. The Effects of solar ultraviolet-B radiation on the growth and yield of barley are accompanied by increased DNA damage and antioxidant responses.Plant Cell Environ.22:61–70.
Metzger J.D.1995.Hormones and reproductive development. In:Davies P.J.(ed.),Plant Hormones:Physiology,Biochemistry and Molecular Biology.Kluwer Academic Publishers, The Netherlands,pp.617–648.
Ros J.and Tevini M.1995.Interaction of UV-radiation and IAA during growth of seedling and hypocotyl segments of sunflower.J.Plant Physiol.146:295–302.
Rozema J., Chardonnens A., Tosserams M., Hafkenscheid R. and Bruijnzeel S.1997.Leaf thickness and UV-B absorbing pigments of plants in relation to an elevational gradient along the Blue Mountains,Jamaica.Plant Ecol.128:150–159.
Saile-Mark M.and Tevini M.1997.Effects of solar UV-B radiation on growth,flowering and yield of central and southern European bush bean cultivars (Phaseolus vulgaris L.).Plant Ecol.128:115–125.
Santos I., Almeida J.M.and Salema R.1993.Plants of Zea mays L.developed under enhanced UV-B radiation.I.Some ultrastructural and biochemical aspects.J.Plant Physiol.141: 450–456.
Stephanou M.and Manetas Y.1998.Enhanced UV-B radiation increases the reproductive e.ort in the Mediterranean shrub Cistus creticus under eld conditions.Plant Ecol.134: 91–96.
Stephanou M., Petropoulou Y., Georgiou O.and Manetas Y. 2000.Enhanced UV-B radiation,flower attributes and pollinator behaviour in Cistus creticus:a Mediterranean eld study.Plant Ecol.147:165–171.
Teramura A.H.1983.Effects of ultraviolet-B radiation on the growth and yield of crop plants.Physiol.Plant.58:415–427.
Teramura A.H., Biggs R.H.and Kossuth S.V.1980.Effects of ultraviolet-B irradiances on soybeans.II.Interaction between ultraviolet-B and photosynthetically active radiation on net photosynthesis,dark respiration,and transpiration.Plant Physiol.65:483–488.
Tevini M and Teramura A.H.1989.UV-B Effects on terrestrial plants.Photochem.Photobiol.50:479–487.
Van de Staaij J.W.M., Bolink E., Rozema J.and Ernst W.H.O. 1997.The impact of elevated UV-B (280-320 nm)radiation levels on the reproduction biology of a highland and lowland population of Silene vulgaris.Plant Ecol.128:172–179.
Yang J., Zhang J., Wang Z., Zhu Q.and Wang W.2001. Hormonal changes in the grains of rice subjected to water stress during grain lling.Plant Physiol.127:315–323.
Ziska L.H., Teramura A.H.and Sullivan J.H.1992.Physiological sensitivity of plants along an elevational gradient to UV-B radiation.Am.J.Bot.79:863–871.
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Yang, H., Zhao, Z., Qiang, W. et al. Effects of enhanced UV-B radiation on the hormonal content of vegetative and reproductive tissues of two tomato cultivars and their relationships with reproductive characteristics. Plant Growth Regulation 43, 251–258 (2004). https://doi.org/10.1023/B:GROW.0000046002.39513.be
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DOI: https://doi.org/10.1023/B:GROW.0000046002.39513.be