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First Report on RAPDs Patterns Able to Differentiate Some Argentinean Species of Section Algarobia (Prosopis, Leguminosae)

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Abstract

Prosopis species constitute a very important resource in arid and semiarid regions. Some species of section Algarobia hybridise and introgress naturally in areas of sympatry. According to previous isoenzymatic studies these species have high variability within populations. However, the genetic differentiation among species was very low, and these markers failed to provide diagnostic loci for species recognition. Here we analysed by the RAPD technique natural populations of Prosopis alba, P. ruscifolia, P. nigra, P. flexuosa, and P. vinalillo with the purpose of obtaining markers for species and hybrid identification, by analysing the distribution of genetic diversity within and among species. Genetic variability (H= 0.12–0.26) was similar in all populations. Genetic differentiation among populations (F ST= 0.39) was highly significant. Hierarchical analysis of genetic structure performed by Wright (1978) method and analysis of molecular variance (AMOVA) indicated that the diversity among populations within species is low (4–13%) and most genetic diversity (54–61%) occurs within populations. The differentiation among species is intermediate (26–42%) between the previous components but highly significant. Five bands provided a tool for identifying any of the species studied, with the exception of P. vinalillo. The difficulty in diagnosing this species is discussed in reference to the hypothesis of its hybrid origin.

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References

  • Archer, S., 1990. Development and stability of grass/woody mosaics in a subtropical savanna parkland, Texas, USA. J. Biogeogr. 17: 453–462.

    Google Scholar 

  • Archer, S., C. Scifres & C.R. Bassham, 1988. Autogenic succesion in a subtropical savanna: conversion of grassland to thorn woodland. Ecol. Monogr. 58(2): 111–127.

    Google Scholar 

  • Bessega, C., L.I. Ferreyra, B.O. Saidman & J.C. Vilardi, 2000. Unexpected low genetic differentiation among allopatric species of section Algarobia of Prosopis (Leguminosae). Genetica 109: 255–266.

    Google Scholar 

  • Bessega, C., B.O. Saidman & J.C. Vilardi, 2000. Isozyme and RAPD Studies in Prosopis glandulosa and P. velutina (Leguminosae, Mimosoideae). Genet. Mol. Biol. 23(3): 639–648.

    Google Scholar 

  • Black IV, W.C., 1996. FORTRAN Programs for the Analysis of RAPD-PCR Markers in Populations. Colorado State University, Ft. Collins, CO.

    Google Scholar 

  • Burghardt, A., 1992. Prosopis L. Caracterizació n electroforética de sus especies. Tesis Doctoral Departamento de Ciencias Bioló gicas. Universidad de Buenos Aires, Argentina.

    Google Scholar 

  • Burkart, A., 1976. A monograph of the genus Prosopis (Leguminosae subfam. Mimosoidae). J. Arnold Arboretum 57: 219–249.

    Google Scholar 

  • Chalmers, K.J., A.C. Newton, R. Wuagh, J. Wison & W. Powell, 1994. Evaluation of the extent of genetic variation in mahoganies (Meliaceae) using RAPD Markers. Theor. Appl. Genet. 89: 504–508.

    Google Scholar 

  • Dellaporta, S.L., J. Wood & J.B. Hicks, 1983. A plant DNA minipreparation: version II. Plant Mol. Biol. Rep. 1: 19–21.

    Google Scholar 

  • Excoffier, L., 1993. WINAMOVA. Genetics and Biometry Laboratory, University of Geneva, Carouge, Switzerland.

    Google Scholar 

  • Excoffier, L., P.E. Smouse & J.M. Quattro, 1992. Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131: 479–491.

    Google Scholar 

  • Felsenstein, J., 1993. PHYLIP (Phylogeny Inference Package) v. 3.5c. Department of Genetics, The University of Washington, Seattle, WA.

    Google Scholar 

  • Ford, E.B., 1940. Polymorphism and taxonomy, 493 p. in New Systematics, edited by J.S. Huxley. Clarendon Press, Oxford, UK.

    Google Scholar 

  • Huff, D.R., R. Peakall & P.E. Smouse, 1993. RAPD variation within and among natural populations of outcrossing buffalograss (Buchloë dactyloides (Nutt.) Engelm. Theor. Appl. Genet. 86: 927–934.

    Google Scholar 

  • Julio, N.B., 2000. Estudios alozímicos sobre variabilidad, estructura y diferenciació n genética en Prosopis chilensis (Leguminosae, Mimosoideae) y especies relacionadas. Tesis de Doctorado en Ciencias Bioló gicas, Universidad Nacional de Có rdoba.

  • Karlin, U.O., R.O. Coirini, L. Catalán & R. Zapata, 1997. Especies arbó reas y arbustivas para zonas áridas y semiáridas de américa latina, pp. 63-71, in Serie: Zonas áridas y Semiáridas No. 12 OEA. Programa de las Naciones Unidas para el medio ambiente, FAO.

  • Kiel, M. & A.R. Griffin, 1994. Use of random polymorphic DNA (RAPD) markers in the discrimination and verification of genotypes in Eucalyptus. Theor. Appl. Genet. 89: 442–450.

    Google Scholar 

  • Lynch, M. & B.G. Milligan, 1994. Analysis of population genetic structure with RAPD markers. Mol. Ecol. 3: 31–99.

    Google Scholar 

  • Miller, M.P., 1998. A Program for the Preparation of AMOVA Input Files from Dominant-marker Raw Data. Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ. mpm2@nauvax.ucc.nau.edu.

    Google Scholar 

  • Morello, J., N. Crudeli & M. Saraceno, 1971. Los vinales de Formosa (Rep. Argentina). La colonizadora leñ osa Prosopis ruscifolia Gris. Serie fitogeográfica No. 11. INTA.

  • Nei, M., 1973. Analysis of gene diversity in subdivided populations. Proc. Natl. Acad. Sci. USA 70: 3321–3323.

    Google Scholar 

  • Nei, M., 1977. F-statistics and analysis of gene diversity in subdivided populations. Ann. Human Genet. 41: 225–233.

    Google Scholar 

  • Nei, M., 1978. Estimation of average heterocigosity and genetic distance from a small numbers of individuals. Genetics 89: 583–590.

    Google Scholar 

  • Otha, T., 1982. Linkage disequilibrium due to random genetic drift in finite subdivided populations. Proc. Natl. Acad. Sci. USA 79: 1940–1944.

    Google Scholar 

  • Ramírez, L., A. De la Vega, N. Razkin, V. Luna & P.J.C. Harris, 1999. Analysis of the relationships between species of the genus Prosopis revealed by the use of molecular markers. Agronomie 19: 31–43.

    Google Scholar 

  • Ridley, M., 1993. Evolution. Blackwell Scientific Publications, Oxford, UK, 670 p.

    Google Scholar 

  • Roig, F.A., 1993. Aportes a la Etnobotánica del Género Prosopis, pp. 99-121 in Contribuciones Mendocinas a la quinta Reunió n Regional para América Latina y el Caribe de la Red de Forestació n del CIID. Unidades de Botánica y Fisiología vegetal, IADIZA.

  • Rossetto, M., P.K. Weaver & K.W. Dixon, 1995. Use of RAPD analysis in devising conservation estrategies for the rare and endagered Grevillea scapigera (Proteaceae). Mol. Ecol. 4: 321–329.

    Google Scholar 

  • Saidman, B.O., 1985. Estudio de la variació n alozímica en el género Prosopis. Tesis Doctoral, Fac. Cs. Exactas y Nat., Universidad de Buenos Aires, Argentina.

    Google Scholar 

  • Saidman, B.O., 1993. Las isoenzimas en el estudio de la variació n genética y las afinidades entre especies de Prosopis. Bol. Genét. Inst. Fitotéc. Castelar 16: 25–37.

    Google Scholar 

  • Saidman, B.O. & J.C. Vilardi, 1987. Analysis of the genetic similarities among seven species of Prosopis (Leguminosae: Mimosoideae). Theor. Appl. Genet. 75: 109–116.

    Google Scholar 

  • Saidman, B.O. & J.C. Vilardi, 1993. Genetic variability and germplasm conservation in the genus Prosopis, pp. 187–198, in Nursery Technology of Forest Tree Species of Arid and Semiarid Regions, edited by S. Puri. Winrock-Oxford & IBH Publishing Co. PVT. Ltd., New Delhi, Bombay, Calcutta.

    Google Scholar 

  • Saidman, B.O., J.C. Vilardi, S. Montoya, M.J. Dieguez & H.E. Hopp, 1998. Molecular markers: a tool for the understanding of the relationships among Species of Prosopis (Leguminosae, Mimosoidae), Ch 21, pp. 311–324, in Tree Improvement: Applied Research and Technology Transfer, edited by S. Puri. Science Publishers Inc., USA.

    Google Scholar 

  • Saidman, B.O., C. Bessega, L.I. Ferreyra & J.C. Vilardi, 1998b. Random amplified polymorphic DNA (RAPDs) variation in hybrid swarms and pure populations of Genus Prosopis (Leguminosae), in International Foundation for Sciences (IFS), Recent Advances in Biotechnology for Tree Conservation and Management, Proceedings of an IFS Workshop. ISBN: 91 85798 460.

  • StatSoft Inc., 1995. STATISTICA forWindows (Computer Program Manual). StatSoft, Inc., Tulsa, OK. http://www.statsoftinc.com.

    Google Scholar 

  • Swofford, D.L. & R.B. Selander, 1981. Biosys: a FORTRAN program for the comprehensive analysis for electrophoretic data in populations genetics and systematics. Heredity 72: 281–283.

    Google Scholar 

  • Vicario, F., G.G. Vendramin, P. Rossi, P. Lio & R. Giannini, 1995. Allozyme chloroplast DNA and RAPD markers for determining genetic relationships between Abies alba and the relic population of Abies nebrodensis. Theor. Appl. Genet. 90: 1012–1018.

    Google Scholar 

  • Vilardi, J.C., B.O. Saidman & R.A. Palacios, 1988. Muestreo segú n variabilidad, pp. 119-124, in Prosopis en Argentina. Documento preliminar elaborado para el I Taller Internacional sobre Recurso Genético y Conservació n de Germoplasma en Prosopis. Fac. de Cs. Agropecuarias, UNC-FAO, PIRB.

  • Welsh, J & M. McClelland, 1990. Fingerprinting genomes using PCR with arbitrary primers. Nucl. Acid Res. 18: 7213–7218.

    Google Scholar 

  • William, J., A. Kubelik & K. Livak, 1990. DNA Polymorphism amplified by arbitrary primers are useful as genetic markers. Nucl. Acid Res. 18: 6531–6535.

    Google Scholar 

  • Wright, S., 1951. The genetical structure of populations. Ann. Eugenics 15: 323–354.

    Google Scholar 

  • Wright, S., 1978. Evolution and the genetics of populations, in: Variability within and Among Natural Populations. Vol. 4, University of Chicago Press, Chicago.

    Google Scholar 

Download references

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Authors and Affiliations

  1. Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina

    Laura Inés Ferreyra, Cecilia Bessega (Fellow of CONICET), Juan C. Vilardi (Member of Carrera del Investigador Científico, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) & Beatriz O. Saidman

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  1. Laura Inés Ferreyra
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  2. Cecilia Bessega
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  3. Juan C. Vilardi
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  4. Beatriz O. Saidman
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Ferreyra, L.I., Bessega, C., Vilardi, J.C. et al. First Report on RAPDs Patterns Able to Differentiate Some Argentinean Species of Section Algarobia (Prosopis, Leguminosae). Genetica 121, 33–42 (2004). https://doi.org/10.1023/B:GENE.0000019925.06080.c7

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