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Differences Between two Subspecies of Dolly Varden, Salvelinus malma, Revealed by RFLP—PCR Analysis of Mitochondrial DNA

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Abstract

Genetic differences between two subspecies of Dolly Varden, northern Salvelinus malma malma and southern Salvelinus malma krascheninnikovi, from rivers of eastern Russia were studied. Mitochondrial DNA was analyzed by restriction fragment length polymorphisms (RFLP) performed on products amplified with polymerase chain reaction. Three adjacent segments (approximately 7670 bp), comprising 47% of the mitochondrial genome were used: two encoding the five complete NADH dehydrogenase subunits and the other the cytochrome b gene and the control region (D-loop). Total composite haplotypes 46 were found among 136 fishes using RFLP analysis with 14 restriction enzymes. The amount of nucleotide divergence between haplotypes of two subspecies of Dolly Varden was estimated to be approximately 4%. The differences in the level of nucleotide diversity, mismatch distribution between haplotypes, and population-genetic structure of two subspecies of Dolly Varden suggest that these two forms have existed separately for a long time.

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Authors and Affiliations

  1. Institute of Marine Biology, Far East Branch Russian Academy of Science, Vladivostok, 690041, Russia

    Alla G. Oleinik, Lubov A. Skurikhina, Sergei V. Frolov & Vladimir A. Brykov

  2. Institute of Biological Problems of the North, Far East Branch Russian Academy of Science, Magadan, Russia

    Igor A. Chereshnev

Authors
  1. Alla G. Oleinik
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  2. Lubov A. Skurikhina
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  3. Sergei V. Frolov
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  4. Vladimir A. Brykov
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  5. Igor A. Chereshnev
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Oleinik, A.G., Skurikhina, L.A., Frolov, S.V. et al. Differences Between two Subspecies of Dolly Varden, Salvelinus malma, Revealed by RFLP—PCR Analysis of Mitochondrial DNA. Environmental Biology of Fishes 69, 449–459 (2004). https://doi.org/10.1023/B:EBFI.0000022897.26755.da

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